XB-CLONE-4354659
Clone Name: IMAGE:7026792
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Gene:
krt7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782856
[+]
taattaatcccccccccccccgactggatctgtctctcagaccatcagcagcagccttgcacctatcctgcactctcctgcactactactccaacctctc
actgagcaccatggctttctccagcagcagacaaaccagcttcagtacccgctccgcagtgcccgctgccagcttcagccaaatgcgcatcagctccact
cgctcctccagcggaggcgcagggctcggccgggcagggggaagcttcagcagctccagtctgtccaacatcggtggcggcgtcagaaagagcgtctctt
acggcgttagcagcggccgctccggtggcgcaggattcagtggcgctgggtttcggggcgcaagatccgttggcgcatgattccgtggcgctgggttcgg
tggtggaatagctttattccggacctgggatccactattttctaaaagcggccccccaccgccggtgggaactttggccctgtggccccaactttggatt
tattgccggccttaataaaggggttcaaaaaaataaaaggcaaattgtcatcccactaatttttcctcaaaaattaataacccttttttcttttcaacat
ggcatatactgaaagtttgtggaggtttatctaccaaaaacttccaatgtacaagtggtaattctatactatcctatgcgtttataggagaaggtggagg
agaaaattaatttttcggtgcgtgataagcacccctcgatgggtaggtgcctggaaataaatgataactctaactgggataaaaaaanatatgagaaaaa
ttttgttggagttaatataggggatatatccgtttcatgggtaaatgggggctcattaaaaaagtaaaaacctcgataacaacgatttagacatctataa
aaaaaatagcaaccacatacataacaagacagggttattaatcaaataaatcatctctcccaataggcactaactgaccacagcgagatagagaagaaaa
taaaaatatatatacatcagaaggctattctgacactcttaccatcactaattatcaaaagacacaccttctataaatactaaagcaatgagcaagaaat
aatccactaccaagaggnaattctcctttcaacctcaggcatacaaaacaacaatacgctccataaggagcacatctaggcgcttctgcacagtcataat
tgatatataatatttacccttatcactatacatctaatcnctcacttatagtaattntacaaaaatgagtagactgatgactggttttagataccctaga
cgcaanttacttaatctcaata
3' EST: CF782855
[+]ggtccggaataagctactccaccaccgaacccagcgccaccgaatcctgcgccaccgaatcctgcgccaccgaacccagcgccaccgaatcctgcgccac
cggagcggccgctgctaacgccgtaagagacgctctttctgacgccgccaccgatgttggacagactggagctgctgaagcttccccctgcccggccgag
ccctgcgcctccgctggaggagcgagtggagctgatgcgcatttggctgaagctggcagcgggcactgcggagcgggtactgaagctggtttgtctgctg
ctggagaaagccatggtgctcagtgagaggttggagtagtagtgcaggagagtgcaggataggtgcaaggctgctgctgatggtctgagagacagatcca
gtcgggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: