XB-CLONE-4354657
Clone Name: IMAGE:7026791
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Gene:
gjd4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF722982
[+]
taattaatcccccccccccccagagaggcgagtgtaggacgcagccagaagcctccgtgtatctcccctgtcccttgcagacatgtaatgtgtagggcaa
atctgtcctgccaggaaactgatgatttactccatggaaccttcattcatggcttgcgtttccttttatctgttgtaaaaaaaacccctcttgttatcgg
gagcgaggcgacattggccaccatggagtatttagactcagtgggatatttaatcatcacttttaactacaatgttactgcaattgggaaggtctggctc
acattaatgcttttgctgaggatggctatggtgttatttgccggataccctctttatcaagacgagcaggagcgttttatctgcaacaccttacaagccc
ggctgctccaatgtctgctacgacatcttctcccctgtgtcccaaatgaaattttggcttttccagaccatgatagtctttctaccctacgctatgttcg
ctgttcaaatttttgcaccggagtgggtgggcatactatgggcaccaaacaaaatggaattaactatcccggggaaaaaggcaatcaaccccgcggcctt
ttttcatttgggagtgtgggagcctttaaatgagatagggaagaatgtacgccggaaattttttttccccttcgggcgcgttaaattataacatgtccac
taatattatcattctttctttggaaaagatcacaatctctctttgtatattataaaaaagggggacctggggggtatcttttttccgcgaagccaccaac
gctgagggggggggtccaaagaagtgaaaaatttattatttcccccgcgcgtctcttgtagggggggggaatagtaattctgtttgtgctttctcacctc
anaatataagtggtaaagatgtttttcctcggctagcgtgagttctttcaaccacaagnaagggaccaccccccccccggtgaggggggaacataaaaga
aagncgagtggggaggtagggataaaaaggggcgggaataagcacccattaacatacggtgaaagtacaaacccacacgactggatgataaaaaaaaaga
caccattccccaaccgctgagagagcgacaaatacaacgtatatatgcnacacggggggggggggggaacacgaaagggtaagggtagagaatattattt
cattcaacaccaaaaaagatattgttgtgagactttgtgagggggggaaatacacccttcccatatattttatctatataaantccccaacttccactng
acggaagaaaaacggtactttgaggctagtaan
3' EST: CF782857
[+]tttttttttttttttctaaatgttataatattaataatgtttatgaaaatgttctataaccaaaacacattgctaaaacaggacaaaactctaactataa
acactgagcgtttagactctgaaacatcaatcacaaaacaatgccaacatttacccactcattaaacccactcagatttcttggatttggacgctgggga
tttttcaggggggctgaatattcccagattatagctgctgcaactcttcctgcttttggtttgagctgccttggggtgaccaagtggtgatcacttcttt
ggtgggtttgccgttcggccagaaatcaaggctgaactttgccaaagaacatggattttattggctgtttgggtgtttcatggttggaaacttttcccca
ctttcttggttgaagaaactttttctgtccggaaaaatgaaagcccgtttttcccctttttatcccaattgggcccaaaacaactgggtaatttcccccc
ccttaaattttctcccccctttctaattttttttttaaagaaccctttttgtaaaaacccaaaaattcaaccgcgtaaacaggggccctatttggggttt
ttcccctaaatcccaccctctttgccaccaaatttttagaaatttttcccgtaagcccggtaaatatttaaaaaccatgtcccaatccccttcccctctt
tttccacccggttttacccccccctttttttcccctcgtgtacaaaaacaacacggcgtcccctctccccctcatttcatgaggcgcggctttttttgaa
aagcaccctcccttttattctaccccatataatgagggaaatattaccggttcttccccgcctttcgtgggggttcccccaaatacgtgtcccccataaa
tatgttttttcctttgtcggacggaaatatattttctcaccctttcttcctagaggggggctcttttcaccggtgtggttttattatacgtgaacgccat
tttcccctcccccttggggttttaattacacactcctccttttttctccccacgcgcgggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: