XB-CLONE-4354654
Clone Name: IMAGE:7026790
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Gene:
rpl10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782859 [+]
taattaatcccccccccccccgctttctggtgttggcgaggtgcagccgccatcatgggccgccgacctgcccgctgttacagatactgcaagaacaagc
cgtaccccaaatcccgtttctgtagaggagtcccagatcccataatccgcatctttgacttggggcgcaagaaagcaaaggtggatgacttcccattgtg
tggtcacacggaatctgtagaatatgaacaagctctcttctaagactcaaggaatctgacagaatgagtgacaacaagagaaagttgaaagaatgagaaa
atggagcgtttgaaaacatggtttgtctgcatatatttattgtaatcgtaatcaacaagatagttatctggggccagagactgacgaacctcataccaca
aatgcaactttattttttggcccagtctcattaataatgtatctcatctcgagcacgttaagaaattacggcggagaaggcgtacggagagagatttttt
cttaataaaattaatgtactacagagaagaaaaaggggaggaggagacaggggggtgggtggtagggagggggtggggggggggcagggggaaggtaggt
tgtggccgtatgagttttaacacagaaaaggataaattttgaaacctattaagaaaagtagtgtgccctaacacaaaacttgtgaataaacgactaaaca
attaccatggctaagatacgacacatacaacaggtacaccaacactcactgtgtggaatgattagttaaatggattagggatagaatgcaaggaatatag
aggttattnttgtgatttatttaaacatgaggttatanggtaaatgcctgtgtttggatgtggctagacagaggaatgnccaaacatanncggaatgtat
tgatgaggtaatgtacttcagattatgtngaaaagccaccgtagangaataatgnggggagaacnancgancacttattccctccaccacccnccaanaa
aattaaaatacaaaaatattttatattttaccatactacctgtaagaatgatcgacagaatattgtagaacaatcgaaactataaaagagtcgtgaatat
ataattaaaagttagccttatagatagtaaatnatatattaaaaaccatgaccgcagattggaaaataaaaacaacncttcccataaacactaattataa
atctgcacttttaaaaaacttatgtgctgtgtcaaacggcagagagttaaaaaaaaataaaagaaatatagaaattttaacgtaccgctggtgaatagat
tgctttgntcatgaaaaattagtantcctatataa
3' EST: CF782858 [+]tttttttttttttttcaacatccaataggttttattgggagagaggctctgctagaagtagaatctaaacagcatgaattgctctccatttgtccagggg
gcccctgttgggaatgtacttcaccccacagccgtcagggatcagccgcttctccgcaagcagagactcaaactcgtcagcgttgaacttggtaaaaccc
catttcttagaaatgtgaatcttctgacgcccagggaacttgaacttggctcggcgtaaagcttcaatcacatgctccttattttgggtcttggtgcgga
tagacatgatcacttgaccaatgtgcacacgtgccactgtgccctggggtttgccaaaagaaccacgcattccggtctgaagtctgtcagcaccggcaca
tgataacatcttgttgattctgatgacctggaaaggatgcaggcaaaccccgaatgtggaaaccctcccttgccgcacctcttacacagatgaacttgtt
tggcaaaaaatacggggaagcttttcaaaaaccttctgaaagaaaagcagatccatcgttcttcatgaaagccgaggtggaacaacccatggggagaaat
acatcataccatttggctttacctgcgacgccctaaaagccaaacaaagacgcgaatattctgggaaattcggggcaagcccactctccaaataaaaacg
gcagaagtcgggaggacaacaggatttggtttaatttgataaacttaagatataatcacactcaagcatacgatcatgtcgagggcacccaatgttagag
gtaggagttgtgcatatcattcgcgtcaagataactaatgaaacaactcgatggtagggggcgcgtagctacactaatatcttaacacactcaacgacgc
gttcgatacgtatatgagtcgtg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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