XB-CLONE-4354651
Clone Name: IMAGE:7026788
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782861
[+]
taattaatcccccccccccccgacccagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgagg
agactaccgccttggtgtgcgacaatggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtcg
cccccgtcaccagggtgtcatggtgggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggtatcctgaccctgaagtaccca
attgaacacggtatcatcaccaactgggatgatatggagaagatctggcaccacaacttctacaatgagctgagtgtggcccctgaaggacaccccaccc
tgctcactgaagcaccaaagaaacccaaaggtgcaccgtagaaaagaatgacccagatacatgttttgaaaaactattaatggtccccaccccagagtta
tagttggcgcacccaaagacttgaagccgggtcacctggttaaagcttacaggggttcgttgccttcagggggtatccgcttccttttggacatgcccgg
gggatgataggggcggatccaccccacacaaaaggagtctccccttagttaagtgttaaagtgatttatgcgcccacctggcccagcaaatggatcaaat
tccattgtgccgagacatgtaggaatccccgagggggacgggagaccatatgagataaccactcgaactcagacagagaattaaagctccccatccgcgt
ggggaataaacgaatgacccgcgcgtgacgctttgaataagaacggcggaagagattcaattgcgtcacctctttttttgtggattccgagacctcactc
nttgaacctggaaaaataatacggtcgggaaaaagacaattcggtgtccctgtgcggagatccaggttaacgaggggagaaaaaaatagaaccgtgtgtg
tgttgttgtatggttgaagcgggttttaccattgctgatatgcttttattatagagcnnagaaaacgtggtatatattgtaggaggaaagaaccttgatt
tatcaanatgtttatactttgtttctctctcagatcctcaaggggcgtgtccgctacacgacatgaagtagatgttttgtggtggnttgtgtaatgatcg
aaaaacgggggtaaaaggggtgacagtaatttagctcaccagattgcgttgagatataagtatttgaattaattttttctactatgtgagtgtgga
3' EST: CF782860
[+]ttttttttttttttttttttttaacgtaaccgcttgtgttttatttagaaagtaataaatatgatggagtgtaaacaaactgtacagtgactgtccaggt
cttgttttcttcgactttgaggtactttgtatgtgttgcttggaggagtgtgcgtaatgtttagaagcacttcctgtgcacaatggaggggcctgcctca
tcatattcctgtttgctgatccacatctgttggaaggtggacagggaggccaagatggagccaccgatccagactgagtatttacgctcaggtggggcaa
tgatcttgatcttcatggtgctgggagccaaggcagtgatttccttctgcattctgtcagcaataccagggtacatggtggtaccaccagagaggacatt
gttggcgtacaggtccttgcggatatcaatgtcgcatttcatgatgctgttgtaggtagtttcatggataccagcagattccataccaatgaaggaaggc
tggaagagggtctcagggcagcggaaacgttcatttccaatggtgatgacctgaccgtcgggaagctcatagctcttctccagggaggaagaagaggcag
cggtggccatttcattctcaaagtccaaagccacatagcacagctttttcttgatgtcacggacaatttcacgttcagctgtggtgacaaaagagtagcc
acgttcagtcaggattttcatgaggtaatctgtggaggctctaccagcccagtccagacgcatgatgggcatgggggcagggcataacctttatagatgg
ggacattgtggggtgacaaccatcacccgga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: