XB-CLONE-4354646
Clone Name: IMAGE:7026786
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Gene:
mettl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782864
[+]
taattaatcccccccccccccatgctgttctgcgcctagcttgagaagggtggctgcctagagtctgtagaaccatgtctaatagccaggcaatgccttc
cgatcctggtataaccatgtgtaatacccaggcaacgccttccgaccctggtattaccgtggcacatcccaagaagcgctattacaggcagagagctcac
tctaacccaatggccgatcatacattccagtacccagtgaaacctgaaataatggactggtcagaatactaccctgattatttcaaaccattggtaccag
acagtgcccatgatgatgccaaggatttacaagagagaaaggaacagcatcaggtggagtttgcagacatcggctgtggatatggcggtctgttagttga
gttatcacctctattcccaaacacgcttatgctgggactggaaatccgagtgaaggtttcggattatgtgcaggacaagatcaaatctttgagagcttcc
catctgggtcagtaccagaatattgcctgcattcgtagcaacgccatgaagtacattccccacttctttaaaaaaagggcagtttgaaccaagaagttct
tttttaattccctgaatccccacctttaaaaaaaaacaaaccccaagtggggaggattatttagttccttacttcggcttggcaaaaaataatcccatag
gtgtctaccaaatttggggggtaatggggtttacaccttataataaaatgtgggaaaaaagtttcaccaccgtggaaggggtaaaaccatttttcccccg
aatcaatcctcctttttttaatcaaattttcacataataaaaaacctggacaaaacaaatatttgtccacgggagatacattgggttcatctccacatta
ataaaaaggaataaaagggccccaaaaaaacacaaaggggtttataacctttttgggcatattttttccttccctccaaaaaaaaagatcccctttttat
ctcatagg
3' EST: CF782863
[+]tttttttttttttttaatcatattaagggaattttaatttccataagatgaaacttatatgaacaaaattattaccaaattttctatttcctgatagttc
cctttaaatacacagcaacctcatttcatgcatttgttttgttcaaaatactggcttaatgctcaagcacagtaaatacagaagtggagatatctataac
agcactaattctgcacacaaattaaatcctgccaataaaaatgctctgtataaattgtgtaataatttagaagacaagatcattggtctgtatctgcctc
ttctgctgcgccccctttttatattgtgcttggcagattctgtaatgtataacacaaaatgacctaaagaaaaagttctagattgctcagtccatcagat
tttcattggaaacataaaacaagtcaggttagagatatcatccatcctactgctgggagtctctttgtataaacgtactgttttctatgcggcgaaatac
tgccaaaaggttcagccctttgtttctctggaccttttttccttcttctgtggaggtacccagcttccccacgacaatatcgttgaccagctcttctttt
gcaactcgctcaaaaagaggatgctcggtgaaatgctttaccatccacgtgtgaacctcttccacgtctgttataatgtacaccatacccccaattcgta
acacataagcatattctgccagcagagtaggactaaaaattcctccacttgtgctttgtctttcttaaagtgaggatcaggggaaaaaaaaagaaatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: