XB-CLONE-4354641
Clone Name: IMAGE:7026784
|
Gene:
cirbp
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782867 [+]
taattaatccccccccccccccgcacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatgaagg
aaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtgaag
gatcgggaaagcaagagatccacaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctgtag
atggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggacgcagagggttcttccgtgg
aagcatacgccaaggtggtggtggatacatacgatatggtggcaaacagcctgcttttgaaaacagtaggtggatgttgaccaaagtcgctggcatcccg
gaggattacttatgggcttgaagacaatggaatgcttatgggtaaatcgctttccgggaagggatcctttcaacgggggaataaagtttatggaacatcg
ccatattaacttaaccgcccaaaggaaagttcatgtacttccctgtttttctgttggggaattttcccaagagagattcattaaccccatgtttagcgga
aacgggggggggatatgaggctgattttatttataaatagacgagtaaaattcttttgtcttgtaggggaaggcgcctttttctgtcgcacagtagatat
attatagcgttttgttgtggtgtgagtctttcatttaatataatatcttatctatccctatattgaactgtgctgtgtttcaccactttgtagcttctct
acttctataataatagaaataatatgctccaccgcttataaaagttatatgaagacaagcgggagaagtaaggtccctcnagtgataagatggaaagaaa
gaaaaaacgagcggacgtgtgaggaaaacggaaatagatggagtaggttgattatagctctatgacataaggtggnggctggtgtctggtgcaatgcaac
ccttgctacagggggaggacaaacgcctggcgtggagtagcctcatcngactgag
3' EST: CF782866 [+]tttttttttttttttcttttcacattggtttttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttg
catgtttagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaagga
ggtgtagccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaac
caaaaaatacagcagcttaaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaaca
gcacccatccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggatagga
atatccttgcagaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattaaaaaaactgtcaggc
ctaattaggtctattaaaaaaaaaagtgaacccaagtagatatactacacaaacgattcggccgaaactcccaaaatctttataaatacagcccttggaa
aggaccatcttggatcacgaatggattttactcctggggaagaaaagctgtcaaaactaatcccgggaggaacctccggaaagattccccatgcctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: