XB-CLONE-4354638
Clone Name: IMAGE:7026782
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Gene:
nr6a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782869 [+]
taattaatccccccccccccccggagagccgatctggcaattacagcaacaggagagtccccatgatgctgctgggttcatggtaacatgcccagctcca
tggctgcaccccagcacacacgcactggcatgagatggacacatgggaagatgaccgcgttgatcagagagcatgccttatctgcggggaccgtgccaca
ggactgcattatggcatcatctcttgcgagggctgcaaggggttcttcaagcgcagcatctgcaacaagcgcgtatacagatgcagccgggacaagaact
gtgttatgtcgcgcaagcaaaggaaccgctgccagtactgccggttacttaagtgcctccagatggggatgaaccgtaaagcaattagagaagatggaat
gcccggaggaaggaacaaaagcatcggcccttgtacagaatctcccgatgaaaaaattgagcggataatgtctggaacaggcatttttgaaaaggaaggc
cacatacattcatgggagtaaataatgggtggaacagtgaaccaaaaccttctccctggggaaaatgggaagtcctcaataaaaagcaaaaccaaccctt
ttccccccctgcggtccctacccaccttttccctcccacaatatagggtttacaaggtgggaaactctccanattgtggaaattccgccggctttcccct
ctacagggggaacagccaatttatttttttggggggaaacctcactggggcaccaaatgttgccctctaatttcaaattaaccctttcctcatatatcct
tctttttaagactttaatagcgagacctcccatccctacacgattgtttaatccctaacaccccaggcccccaaaaaaacctttgtaacccggacataat
ccacaattgccttttctttttaccctattttgccctcattggcttagcgaaaaaaccataaaccctccacatgattaacgcacattgtttttttatatga
gcggggccgcgaaaacgtgcacctagcccgaatcttctttgtcattgaacctacccc
3' EST: CF782868 [+]tttttttttttttttggttcccatttttgggctgatactgtctttttttttttctcctctcttcttcggctaatccaagttgcctcaatgcagtttccac
atggctttggcatctcgctcactccttggtcaggctagttttgcatgagtgaaggaaggctttgaaaagcagtgggagttgctccagagggacattcacc
agttttccagcaatgtagcgaacctcgggtaggcacatcatgaggtctgggaacctgttgggctggtgcgggtacctgtactcggtgaagtcttggcaca
cgtaccagtatcgcttgttcagttgttccacctgggaaacactgcttatgccctggatatcttggtttaggaagttgatggctttcatgcagacatactc
ttcgttactaactttgagttggctgaacttgcggaacaaatagatgagccgctccatcacttccattccatcctcactaaacctgtgcaattcgtcctca
gaaggagagtacttggaagtgacgtcggccaagtcgccaaagatctgcttactgtacgtggtgagcgaccagagcaaaatcagctcctgccatgttgtgc
taagcagacatgtgtagtccttgatggacaattccagaaaatggcagcttcttcccccaagtgatctgccggaaaagcaacttattctgcaacccggcac
acaaagggaaacaggctctgaatggggtactttggaacccttccccataaaccttaggcgtaatggaaagggtttatattcccccccccctaaccaactg
ggttaatgaaggtgtggccaacggcggggtcaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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