XB-CLONE-4354636
Clone Name: IMAGE:7026781
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Gene:
hnrnpab
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF722985
[+]
taattaatccccccccccccccgggagagtccatcttggaggaatttggcgattgttggcgcttgtcctttcagtgcatagaaacccctcacaatgtccg
ataccgagcagcaacagtacatggaaactggcaccgagaacgggcacgaagcgtgcgatgctgaggccgcggagagtaaggtgcccgggggccagaacgg
ctcgtaaggcgatcagatcaatgccagcaaaggcgaggaggacgcagggtgcgtacctgatatttcttcccctttaactgaaagagtgaaaatgtttgtt
ggtggcttgagctgggatacaagcaaaaaggatttgaaagattacttctcaaagtttggtgaggtgtctgactgcacaatcaagatggacccaaacacgg
ggagatctagaggttttggatttattctgtttaaggatgctgcgagtgtggaaaaggtattggaggttaaaagaacacatgatttaaatgggtagatata
attgattccaaaagaagggctaatggccatttggtaaaaagggattccctaattaaaagaaaaattatgtttgttttgggaaagggtctttcaaacccca
aaaaaaaatcaaggggaagaaancacactaatattttctacggaggngattgtaaccttttttgggaagtaaccatttttttatggggtaaaaaaaaaat
attttgtggaaagggggtacaaatttttttggatgctcccttttttctcccatcatattgggggggaaaacctccccctgtctgtatacaaaatctatta
aaagttcgctaatgtaaaggtgtaaacagaaagagagagctatttttttgggtgggtggtttatcaaatttcttgacacattgttttttaaataggggga
aaatataaaaggggaaaaacnccaccccgttgtgtgatatgggaaaaagaatttttatttttgtggtgatggttnacttaaaacgtctttgtcttctctc
aaaaaatacgttctttagaaatttgtgggtaaaatacacatggctgatgggtgggaagtaaatctctcatgagatttttggcnccccttaacctctataa
taataaacaatgtgtgtgtatgaacccagcgctcatctcccatttattattgtgtgtttgtgcactaaaaaatgtgatgggcaccacccattttgttggt
gtgtgtaaaaaaaaaaggtgttagtgcctatgatatggcg
3' EST: CF782870
[+]tttttttttttttttcactcaaagtttgttttatttcaaaagctaggatagcttcaatatccatgttatggtgaatcaacacatgtaaaataccttacaa
tacattagattccaaaaggtaccaaaaaggacagtaaaattaacacttccaatactggaaatgtatgacaaaacaatataaaataaaaaggtggaaagtg
acactggttccctaggatgagtcaatctgtacaactggagtaagtccatacttatctccaagaggcagtccctcgatgcaaagttgcagctttaagcaga
gccccaaatctgttcagtttttacctattgattaaaaaacctgactttaagtgttcagtatggcttgtagttactctgatggctcccacgtcttggagct
cttccatagtttgcactgccctgactgtactcatagcctgggccacatccgtaatagccatatccagaattactcatagtttccttagccaccatagcct
tgttggccatagcccccatacccttggtttcccatcccctgattccagtagctgttgtagcctctgactccaatttctggccttcgacttttaaccccct
ctgccagcctcttcccaacaaagcctgccaacccgtggctaccacaatactgttttttgggtacaccttctttttgggtctgttgccccccttggttgac
cacccttggccctcccccccgtatccatggtggaaactcttttttctccccaaaactctccttttatgggccttttccttcctccccactaaaggggggt
gaaatttcccggagcgctgttctccctcccggggattgggctatttggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: