XB-CLONE-4354622
Clone Name: IMAGE:7026775
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Gene:
arpc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF722987
[+]
taattaatcccccccccccccagagggaagtggttctgggcagggagcggcggcctgtcacacactgacaccgagataccgatccgacccctccctgctg
tcccccgccaggtgccgccatgatcctgctggaggtgaataaccggatcatagaggagaccctcaacctcaagtttgaaaatgcggctgcagggaacaag
ccggaggcggtggaaatgacctttgcagattttgatggtgtcctgtatcatgtggggaacccaaatggagacaaagggaaagtcatggtcagtatttccc
tcaagttctacaaagagctgcaggaacacggagctgacgagattctgaagaaggtttatggcaattatcttgtggccccagagcctggttacaatgtgtc
tttgctttatgacctggaggttctgccttccaacaaggactccgtcgtccaccaggccggcatgctgaagcgcaactgctttgcctccgtcttcgagaag
tacttcaagttccaggaggagggaaaggatggagagaggagagccgtgatccactacagggacgatgaaaccatgtacgtagaagcaaagaaggacaggg
tgacagttgtgttcagcacggtgtttaaagatgacgatgatgtggtgatcgggaaggttttcatgcaagaattcaaggaaaggcggcgtgccantcacac
agctcctcaggtgcttttcagcccccggggagccgcccctttgagcttaaggaaaacaaaagccgccggtccgggggaaaaacattgggaatatatccac
cctttggggctcctttttcctcggtcccaccaaaacggcgcaaaacggagaaaaacaaacccccattcaaatttttaatcccccacaaatttccgccgga
aataatcttggc
3' EST: CF782878
[+]tcctgcattgttctgtgccaggggagagcagataccaaccagatcaccagtctgcccgtggcctgggtgccggggatcagcgggcaaatgttttgcccgt
tatagttttcatttctttcttctctgcgtctgggcgggcgcggttcagaaccttaaggaagtcggatgtttttgccctcatgcgagtgtggatgtacgcc
ttggaacatttgatatgatagtgcaagtaatcgcggaatgtgtggataagattgatggtgttgtctctcgtgttggcgttggtgtgacgaggaaagagca
caaaggtgatatatccaatgttgtccccgaccgctgcatctgtgtccttcagctcaaggggcggctcccggtggctgaaaagcacctgaggagctgtgtg
actggcacgccgcccttccttgaactcctgcatgaaaaccttcccgatcaccacatcattcgtcatctttaaacaccgtgctgaacacaactgtcaccct
gtccttctttgcttctacgtacatggtttcatcgtccctgtagtggatcacggctctcctctctccatcctttccctcctcctggaatttgaagtacttc
tcgaaaacggaggcaaaactttttcgcttccgcatgcccccctgttggacaacagaatctttgtttcactgcataacctccaggtcttaaatcaaacgat
cctttgcatccaagctctggtgcccccaatattattttgccatataattattctttgaaagttttcgcctgcttctggggtcttctgcggctttttctct
agcaacctttcgtgaata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: