XB-CLONE-4354612
Clone Name: IMAGE:7026770
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Gene:
hnrnpl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782884
[+]
taattaatcccccccccccccccgagtcccggcggatcactgaagatggcggcagctgcggctgtaggaggaaggccaacatattacgggaacgagggtg
gccgggccccgaagagacagaagactgacgcatctgaggacaacgtggcggcttcagcggaagggtacgatgaccctcacaagaccgcggcttccccggt
ggtccatgtgaggggacttattgatggggttgtggaagcggaccttgcagaggctttgcaggagtttggaacgatcagttatgtcgtggttatgcctaag
aaaagacaggctctggttgaatttgaggatattcttggtgcatgcaacgctgtaaactacgcagcagacaaccaaatctatgttgcagggcatcctgcat
ttgtaaattattcaactagccaaaagatctcccgtcccacagatacaggcgatgattcccggggggtcaataatgttctgctctttaccattctgaatcc
aatctattccataactacggatgtgctgtataccatctgtaatccttgtggacctgttcagcggatagttattttcaggaaaaatggagtccaggccatg
gtggaatttgactctatgcaaagtgctcagcgtggcaaagcttctctcaatggggctgacatttactctggatgttgtacactaaagattgaatatgcta
agcccttctcgactgaatgtctttcaaaatgaccaggaatcatggggattacacaaatcctgcccttgagtgggacaaggtgattgctgcagggttattc
ccaacaaaccgaacagagaaaccccaccacctgtttagggtggaccacccctggccgaaaatatggggggttccttcagggcagggctaatcatggggtc
ttttaccacggaagggaaggcttattggtgctccccccaccc
3' EST: CF782883
[+]tttttttttttttttgtgcatcaagccaggatggctttatattttatcttgctaaaaatttgtcatttacaaaccacacaaagcaatgttctcaaatgtt
ttattgatatgtgcaagcacatcatcatagcaaaaaaaaaaaaaaaataaaataggaagtcacaacaatatttcagaacataaaaactagctccacaagg
aaccaaactaatactaaacatcaaaatcagggctaggaaatcagacaggtcttcttaggaggcatgctgagcagtggagaagcagagttttagagtgtag
gggtaaggaccatttggatttttcatctggtagtgattcatgagaccgagagtctccagtgcttcgctcttagaatcccactccagcagtccggaagagc
ttcgctcactctttccagaaaagattttgattgaagctggcttcttaacacccagttcatcacacatctctataaagttctcctctgtcacctcaggagg
agcattaaagaaatgaagcacactgctcggctgttggattctgttcttggcagcttgctctggggatgtaaatctgttgttacgggacccactgaagact
ttaaaactgcaggagccatcttccaagccataagactgcccaggcacaaaagactgttgctttgaaacacagacacttagtttttgcccaaacatgaagt
tgttgtttaagtgtgtaatggcacggtctacagcatatccatcagccatctccaccatagcctgctcaaggttaactcttcaggaaattttacccttccc
cacattaaccataacaggcagaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: