XB-CLONE-4354607
Clone Name: IMAGE:7026767
|
Gene:
reep5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782887
[+]
taattaatcccccccccccccaagcagttaaattcccctaaaacaaggagggtcccatataagcagctaactattataggggactgctatcatttgtacc
tctcatgtgacgcacagcctgaaagccattgcagcacatccatggcagagctctggcattcaggctacgctggcaccaaggggtttctattgttgccttg
gataccttttttgatatgaagaaagaatgtgttgacgtagttgtactatgttgtgctgttgtctgtagagatgactgagattnttatttaacagcagtca
gcagtgcccagagctgggccactcggccatcctgctctgtgcgggaaagacttttaacatgtattgtctcatcctgcatccagtgagctggaaaaaatga
agtacttgtcaaaanaaaaaaaataaaaaaacacacacagggacagtggctagttataaaaaaaaaaaaaaaataataaaaaaagaaaataaaaaaaagg
gagggacccttttttttctaaaaggggcccccccccccggggaagaattgtgcctgggcccaacattttttttttttggcccttttaaaggggtttacaa
aaaaagcgcagcgcgcctctccaaattttttccacaaaaaaaagaaatttttttttttccccggcgaaatctaaaattggggggtggtggtccaaaaatt
accccaagtgatgttccttacaaccatttccggcaaacaaggaaaataattatatcgggccagaacacctcaggggggcgtagaaaaaaaaccccttaag
aaaaaaaaaacaaattggttataaaaacacccctctctagaaggagggagagaaaaaaacacacctgcttggaaaaagtgtggcttcaccatacggcggg
ggggggaagaaaaccccccagggttctcccccaaaaaataatgataaaataaccacacaaattgggctcattctctcaatttatcaccaccatacccacc
tggtgtgggaaagaaaacctcc
3' EST: CF782886
[+]ttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttgggaaaaaactttt
ttttttcccccccccggggggggggggaaaaaaaaaaatggtaaaaatttttccccccaaaaaaagggggggcaaagggccccccccgggggcccgggaa
aggggttaaaaaaaaaaattcagttttctctaagaaaaaaagcccaaaaaaaaaaaaccccgccaaccaatttttttttttaaaaaaaaaaggggtcccg
ggggaaaaaaaaaaccccctgggggcccgggaacccgaaggccaaaaccccccccgggaagggcggcaaagggttttagggggggggccccagggggggg
aaaaaaggaaccccccccccaaaaaaaaaaccggcctaaagggggccccccttgttttaggggaaattaaacggggggggggggnggggggaaaaaataa
cccccgggcaaaaaaaaaaatattttgttttccgggcccgaaaaaaccaaaaggccgttgggccacgggggggaaccctgggggaaaaaaaaagaaaggg
ggattttttctctcgggggcccaaaaccctagggggggaaagcacaaggggggttggttaaaatttggggcgcccgatccaaaaaaaccaaagggggggg
gggcctcaanaacctttatagaggggggaaaaaaaaacacccacttggggcaaagggggaaaacccccctccctagaggaacaaactcaccgcgctctcg
gaaggaccggatataccccccccccgtggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: