XB-CLONE-4354604
Clone Name: IMAGE:7026766
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Gene:
gins2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782889 [+]
taattaatcccccccccccccggccccttcctatcgctgatagcgggagattcagtcatggatgcctccgaggtcgagttcttggctgaaaaggagcaag
tgactgtcattcccaactttagtctggacaaggtctatcttattgggggtgacttgngtcccttttatcctggtttacctgtagaagtccntctatgggt
ggccatcacccttaaacaaggcagaaatgtcgccatgtccctcaccagggaatggatgtcgataaactaaaggccttcaagatcaagaacccgaaaggga
acttttactcccaagcccaggccctttttaatgggaacttttctaaactttttgttaaaactggggggcaaaaatatttcccctaaaagaaaaaagaaaa
ccccaaccctttttttaaaaaaacccttgtgggaaataaaaaaaaattgcaaaaaccccccgggtttctttttgggaaaaaaaatttttttttaaaaagg
ggggggggaggaaatcccttcctcccccaaccggggggaaaaaaccccccggccccccctttggggggaaataattaataacccacatttgggaggagat
gtttttttttttttgttgtggaggggtgctttttaaaaaacccctcagtgtttatagaaaagctatttgtttttacccncgcggcgtggtgggcaaccac
cccttaaaaagaaagagaagaagatgcccccccctcgaatttatttaatacacaaatatgttttgtccctccaaaagaaagggggggggcatattatatt
ctttgtgtgtggaggggggggggtagaaaatatgttgttgttgggtgttggaaaaaaaaaaataataattttttgtctccttgttcttttctgacaaaac
aancttaatttatttttttgggtgggggggagaaacaatacaactagagagggccggggcgg
3' EST: CF782888 [+]tttttttttttttttcttatttttgcatggtgttaaattcatttactctacattgtttataaaaaaaatatataaatcatgtacagaaatttaccttaca
gcttagaaaaaaaaaatgcctacaagcaaaagaataaaatctgttaaataatagctacaattcattacagatgtatggattttggtctacattctctcct
gctgataataaaagttggaatttataatatattggtactaatccatgtgcgattttctttaaagtgatctccacccaaagaaagtatcattctaagcaat
gttcctatattgattaaaaatgccaatagttttaaagttatatgtatgagtaactgcaactgataacaatatatttgtttatacttttcaggagtcagaa
ccaggaatgcagagaatataaacagccaaacagatattgttttcagtagcaaccacatttacaaacaactacaaaaccactgaacatttagtactaatat
acattttaagggtgcatataattaaattttctttcactattgccaaagggtttttaggtaagggccttctttttagccgtacatttaaggacataagaag
gatagccagtaataggccttggggacgggggttggaactgaaaactgctccaaaactcagaacacattttgggccaaaaatatcccttgccaaatgccgg
ctgggaaagggaaaaacaggggatggggtgttgggaaaaacccccccatggaaaacggctgttaaaagcatcggattatggattcttccaaaaagccttt
tgggtttttggaaaccccccgggccacatcaggtttttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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