XB-CLONE-4354598
Clone Name: IMAGE:7026764
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Gene:
tubb3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782893 [+]
taattaatcccccccccccccggtgtccgcatcccgagcagaaccgagtcacaatgagggagatagtgcacatccaggccggccagtgcggcaaccagat
cggggccaagttctgggaagtcatcagtgatgaacatggtattgacccatgtggaaactatattggagattctgacctccagctggagagaatcagcgtc
tactacaatgaagcttcctcacacaaatatgttccccgggcaatcctagtggatttagaaccaggaactatggacagtgtccgctcaggagcctttggac
atcttttccgaccagacaacttcatatttggacagagcggtgctggtaacaactgggcaaagggtcactatacagaaggagctgaattggtggactctgt
gttggatgtagttcgcaaggagtgtgagaactgtgattgtcttcaaggcttccagctcactcattctctgggtgggggtacaggctctggaatgggcact
cttctaataagtaaggtcagagaggagtaccctgaccgcatcatgaacacttttagcgttgtcccttccccaaaagtatctgacacagttagttgaacca
tataacgcaacattttctatccaccagctggtggaaaatacagatgagacctactgcattgacaatgaagctttatatgacatctgtttccgtactctca
aattggctactccaacctaaggggaccttaaccatctggggcatgcccccattgaagtggagtggacacctccccctaaggtttccctgggccagccttc
aattgcaaaacttggcgtaaactctaaccttccaaaaaggggtaacctttttccccccgatctggcgactttctttttaatggccaaggggtttttggcc
acacccctgtacatgtgccccgtggggccaccccccagcccgtattattaaggga
3' EST: CF782892 [+]tttttttttttttttcgaacagcaggaagtttatttatttaaaaataaataattcagtaaaatagaacacatgaaagacaaaacagatagaaatgtaaga
cacaaacaaatgtgtatgtaagacaggggagatctttctgctgcttgagcattatgcggtgtaagtctcagtcggtgctgtaactgttcataactttcta
ttagcacaaaatatcccgtcagattccaagaaaacattatgcgcacatcctacagctaccttcatatttcctactgctctgcctaaaagttaagctcttt
aggggaggaaacctcctctaaaacacaagcatggaggtttatatgcttaaacactagtcaatacaaacctactattatctgccactcctatccttaaaag
ctttctcctttgttagactcaattactatcacaaaaacacttcccctgttgtaaacacctctgctgacgaaacagccaagcatttaaatgttatttaagg
tgcaaaacccttttaagcagcgcctttttagtaaatgaatgtccccccaattttgctcagcaagggtacatccgacccctctgtaaaataaggcatcttt
catctttcgttaccaaccttatgccgcatattaaaaatactggcatttgcctctctgggccagcaaaaaaaaaaattttaaggggaatgggaggaatgga
aaccccagggttttctgaaaactgggaggggaacctataaatcctaaactttaaaagcaaaccctttgtaccatggaagtggaatttccctaaaaccctg
gggggggaattattttctggaaggttccccggggcttttgccaattttttcccttttattttcccccgggaacctttcaaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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