XB-CLONE-4354595
Clone Name: IMAGE:7026763
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Gene:
hpf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782895 [+]
taattaatcccccccccccccggtcatggctggaggcgggaaagggaaaaggaaaaagccggctgctcagcagtgtgaagaaccgtgtgatgcaaaaaag
aggcactcgggcagatcagaggtttcgaaggatgtacaacaagaggtagaggctcagtatcggctcagcatgcctgaggatttctacgagttttgggctt
tttgtgcagatattgattcagaagcaccaagtgatgttctgcagtcaagccttgggttgcaacttgttggcccttatgatattatttcaggtaagctcaa
gataaccaaataggaagccgaaatagactactatttggactgaaagatccaataagatccaccaagaaattcttactaccctggtgggggacagcgagaa
cacagctacaatgaagaatatttaaaggattactcctgaaagatacatgtagaatattgttggagaacatagaaagcgtctagtaggaatggagaactga
atgcaggcgtagggagaagaaaggaagtgtgaggggagggagaatgaacgtgagtgagttttcggaacaggtcgtttaagagaagagtataaaaaataaa
ataacatccaaaatcaaaagaaatgaaattaaggggagacgcgcggaaagatcggggcggctgggagagatggcaatgaaggccagaagagtagagaggg
ggagagcattcaacctcgcgaatcaaagaacacacaaaaaaaaagagcggtgggggggggccccggtgagtgaatacacatagaacattgtgcaanagaa
atanagtaagattacgcggagaaaatcggagggtgagacgggcggttttggtcgttagctcgtggcttttgaacgaaagtgccgtcgcaagccgagtgaa
gggagcaatgtcggactggtgttgggganantgaaacacaaaatcgaaaaagatatagtcggaaacngaaaaacacaacagaaagatagagataatataa
tttaagttagagaaggtttgtatgagtgtaaaaattttaatgataaaaaagtaatagaataacgaaaaaaanaaaaaaaaacttacagcgaagacaagaa
atctctgaacgaaggaacaatgggaacaaaaaatagaaaagagttgagaagagaatgagagaataatatncaaccatataaatattcccatagataataa
atgaagaagacaagaataaagtattatgaaaaagagtgtgtatggtgggtgtaggtcggggggtgagagtttaaagggaaacactagcggagaacggacg
ggaagnatggggaatgaagattcattgtagac
3' EST: CF782894 [+]taacagaagaggtccattccaagttcataccccataccgtaatcacattcatcatttgcaaactgaacataagttatcatctcttggattggagcaaaag
ctttcattcttttctcatcatttggagcttccacaatgttcttgcaaattcgtttcaggtttccatctgtctcaggaagctccctgtacccaacatcatt
cttatcaactggtaccacaagacctgcaccatggaatgactttgttacaactttcttctcccgtcgtttcatggttgcagacttttgctccaacgagtaa
cccaaatcttcagcttgtgcagtcagcttcgctatcagatctttaattgtgctggatagatttctgtctgtaacttccttaagcttgttggaagcatatt
gcttgacagcagaaaatacattatctccaagctgagtaatttgacatcctttagcggcttcattggctccaacaaagacaggaagatcttcaggagagtc
tctgtaataccccatatgaaactgtgttttgctatcccccaccaggatagtctgaaattcaggtggatcataatagaacctccagtgcaaattgtaattt
attttcgcttcctttttggttatcttgtgcctaccagaaataatatcctaagggccaacaagcttgcaacccaagggctttgactggcagaaacatccac
tttgggggctttctggaagtcaataatcctgcccccaaaaaagccccaaaaacattggggaagaaaatcccttccagggcaatgtgtggaagctccgaaa
taactggggggccccttccaatccacttccttgggttagataccatttccccgtctggaaaaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: