XB-CLONE-4354585
Clone Name: IMAGE:7026758
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Gene:
ola1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782901
[+]
taattaatcccccccccccccggtggctgctgagtgtgagatcctgaggccccacgcctgcagtccgggaagccttcctactccgtctggagggacacgt
gttgagcaactaggatgccacccaaaaaggcagacgatggaccaaaacagcaccctatcattggaaggtttgggacctctttgaagattggaattgttgg
actgccaaatatagggaaatcgactttctttaatgtgttgacaaagagccaggcagcagcagaaaactttccattctgtaccatttatccaaacgagagc
agagtacctgttccagatgagagatttgaattcctgtgtcagtaccacaaacccgctagcaaaagttccagccttttctgaatgtggtggatatagaagg
cctcgagaaaggagcaagtacaggaacagggcttaggcaatgcattcttgtcaaacaataccatccgttgtgaaggaatttttccaccctgatggagtgc
ttttttaataatgatgatattcatccgagggttgtgaagaaaacagtgcgcgcccttgttgtcgttgaacggtttggaggatttgtagcaaggtgagaag
acactttcccggccttgtatatgtgtacgcaaaaaaataatctgattgtaatttggtgtttggttcctctctttttgttttaataantcgttttgtggat
gactaaatatgtattgggctcttttacttttaagtcaaagtgggcctcgccgggggtgtaagtctgtctaagatgtaagggttttttgnttcgaaggtaa
cggccttcgggagacaatcgtctcacccagggaaacaccgggtctcaagtgttgtctggtattcccccgtagatttctttcgcggccgccctaaacagcg
ggagggtcgaataacaaaaaaaatgttctttggggttctcactgtgggtcntcttcctcttatgttgcgcaacccaacgactctttttttacccctcttc
tgtagaactggtggcaaattgtttgacacatacggacctataaatcttcgacatccggtttttttaaattttctcctatttttatttaaaattccccccg
gcacacatttaaactaaaatatcagggagaaggatccgcatacatancagtttatccagaacngcctatctaggaatataaagataatattttcttttcc
cccatatatttataagtctttacaggtcgctttgtgagttattttatttatgacggtttgctctc
3' EST: CF782900
[+]tttttttttttttttctttttttttttttttttgtcaaatgttttattgagtgtcgacatctggagtactgtaaaacatgcattatctgtagattccgaa
cggagcgccgcacattgtcctttctgtcagatgtgtcagccttggcatacatgatggagattaatgaactatcatgagacttgggtggttgcagagaggc
ttctgtgctttgcagaagggtcttaatcatctgaaaagcacattgactgggctgctattgctttgcaggaggtgacatattccagttggaatataaacat
ttggttttccttgggggggggggggggggaatttcactgtcttgttattttttgcattctgtctaaattaaaataaaaaacaaaatgtaaattttaccaa
tgaagtagagtttttttttttttatctcccacttctgcttcaaaattaaaaattatttctttttggattgctgaggtgtattaaacttaaaaaagataat
gtctcccgtcttctactgtgtaattcctgccttgttgtccggattttcctggcgccttgacaaatgactctgagccttctttctttaaatcctcaaattt
tcattaacttccgcccaaatgaaaccccttctcaaaatctgggggggattttggcctggcaccccggaggaacccttcctcccctttcttgaagggccca
ggggccgtactttcatccggggcccgggttggtaaaaaaattattccaggtttggaaggggccccactatccgggtttttaacaaatcttgtggaaaaaa
aacccttttgggggccacttttcctcctggaagaatactttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: