XB-CLONE-4354574
Clone Name: IMAGE:7026752
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782908
[+]
taattaatccccccccccccctgagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatatataaataagtgcaagc
aagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaagccctc
agtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaaaccag
ccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaagggtaaaaaggg
agcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggaaaaacaaaaagtgacgaggcccctgcttct
gatggaggcgacaaggaatcaaagtcttgaataacctttcatcttgtaccgagtctttttatcaggggttccttgtacctccccttgtacaattcaaaaa
gaatattttttatccaactaattttggaaaaagcaaggtttttttagtagttttaaattcctagaaaaacaacattttttaaaaatagggagggaaatcc
cccaccatttttgttttggttggtaaaaagaaatttttttttttaaacaccccttaatgattttggtacactttgccccccggtaatgggtttgttaaaa
gggctaatgaaaaatcttaatcccaggtttaaaaattataaaccacccaacccagtttcagggtggggggaagggccaaaccccctggtcgcagttccaa
aaaaagggttttttttacttaaaatttggaacaacaaaaaatcggtgtaggaaacctcctatagggggg
3' EST: CF782907
[+]tttttttttttttttaaagttcagagagaagttgtttcgtagaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttctcg
gccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcacatc
acactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaaata
aaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaacat
gagtacaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcaacctgc
atagagtactagccgttttgtaccaaaataaaaaaaaacattttagactgctcaaggcttgccctccccacagtaactgggtttggggtggaatatttaa
ctgggtatagaattcatagggctttacaaacccataaggggggcaaggtacccaaattcctaaggggttttaaaaaaaatttttttttacaacacaaaaa
atggggggggggaatccccccccatttttaaaaaaggggtttttccttgaaatttaaccttcttaaaaaaacttgtgcattttacaaaaaaaagtttgga
aaaaaaataatttcctccggaaatttgtcccaaaggggaaggtaccgggaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: