XB-CLONE-4354571
Clone Name: IMAGE:7026751
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Gene:
rps12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782910 [+]
taatatcccccccccccccgctctttccacagttgttctggatgtggcaggcgatttgacgccttactcccactcctgatccaacgatggccgaggaagg
catttctgctggaggtgtaatggatgttaacaccgcactgcaagaggtgcttaagactgctctcatccatgatggtctaacacgaggaattcgtgaagct
gccaaagctcttgacaagcgccaagctcacctgtgtgtgttggcttccaactgtgatgagccaatgtatgttaaattagtggaagccctttgtgctgagc
accagatcaatcttataaaggttgatgacaacaagaaaccttggtgaatgggttggtctctgcaagattgatagagaaggaaaaccccgcaaagcggttg
gttgcagttgtgtaattgtcaaggattatggcaaggagtcttcaagccaaggatgtcatcgaataatacttcaagagcaagatatacaccatccaaaaaa
taaaaagtaattgagccctgcaaaaaaaaaaaaaaaataaggatccccactaatttccaagaagcgggccagcccaccaactgaagggaagctattggac
attggtaaccaaactttgttttttttatggccagaatttaaaaaaatggggatataactcatatgagttcagcttagagttagcgcgtgtttcgggaaat
ttttctcgacgagaaatttaagaagggtctctttatattaatatacttttgggacaagatttacagaaaattattgagaaggggagtatttttgttacac
caagaaaataccttcgatacgagtagggttgcgcgtatgccctgtgnagtccgactaggntnccaagggggaattactcgcggtaaagattatgaaaggt
tatcgcgggtgtctctagaacaaaaagaaagtgtggaccgctgggggaacaattaataaccctgttgctatgaaaaaaatgtgagagaaatttcttgtgt
cgtttaaggtgtatatcttcgtaatgtcgagaggggtgtggggaaaaagaaaaatgcaacaccctgagagagtaataatggggcgtgtataccaagttat
atgaagcgctgtttgttgaacagtccacccctcgggggactttctcctcttaacatttgtgcgcagaaaatatagtaggtcgggtngtgggggggggacg
tgccggatcgtaatacttcgtctcgcgagacttatgatgtcatgtcgtn
3' EST: CF782909 [+]tttttttttttttttgcaggctcaatactttttattttagatgtttatttcttgctcttgaagtattcttcgatgacatccttggcctgagactccttgc
cataatccttgacaactacacaactgcaaccaaccactttgcggggttttccttctctatcaatcttgcagagaccaacccattcaccaagtttcttgtt
gtcatcaacctttataagattgatctggtgctcagcacaaagggcttccactaatttaacatacattggctcatcacagttggaagccaacacacacagg
tgagcttggcgcttgtcaagagctttggcagcttcacgaattcctcgtgctagaccatcatggatgagagcagtcttaagcacctcttgcagtgcggtgt
taacatccattacacctccagcagaaatgccttcctcggccatcgttggatcaggagtgggagtaaggcgtcaaatcgcctgccacatccagaacaactg
tggaaagagcgggggggggggggatattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: