XB-CLONE-4354568
Clone Name: IMAGE:7026750
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782912 [+]
taattaatccccccccccccccgatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctatag
gtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagga
ggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaatg
aaaagcacgccatgcataacctcaatgaccgccttgcttcctacctggaaaaggtcagagccctggaagcctccaatgccgacctggaagggaagatccg
cacttggcacgagaagcaatcaggcaccggcctttggacctgggtctaaagactacagctaaatatttttgaatatcatcagtggagctataggggcaaa
aatccatgggagacaactgttcaaataaaccgctaaaggtggaccactggcagaaatcgacaaaccgccccataacgtggtcatggctgaaacgaatctt
ttagaaagttgacatttttgaaagagataaaaccgcgggtatgctttggtgcagcgaagacgcgggggggagtacgagaaacgcccgggtttcctgtgta
aattaatatatctctgggcatatgggcccgagaccctgggagcagtaaaggtggaggcgcggggagagaagggcgggattttggggacaagttggacctg
atttgaacacgggggttctccctcgtactaattaaaatatacattttagttagtaagcagtcctcctcctcgtcccccagagtgctacttatgcactgta
ctgaataatatatgtaacttgcgtgcaaaatttagaattatcgggcgttccttatgtgttan
3' EST: CF782911 [+]tttttttttttttttggagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcaggg
gaaatttctgttcatttgttcctttatctttcccgactgcccttggatttatgacactgttgaagaaacaaccttcccatctaccacctcttcgacaatg
gtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttccc
catccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggaactgcttgtactcccatgttctgaagttccatgtccgacc
tgatttgcaaaaacttgttcctctagggctggctgatagtgttctgaattctgtaaaaatttcgccttaatagaaacccttgtatttcattggaggttgc
cccttcaactggattagttttcaattgccaagtttggaattgtcatctccaattttttcaaatcatcttgaagtggacacgttttttccctcagatgaat
attcaccctcgttgcggggcagctgaaactttgtatcaaacccccaacgtgagaatatatttctttttttaaaatatccaaggccttcattcttggggtg
agaataaagaaaaactcggtggtccccgtggcggagtatttctggaaaccctgataatttttataatctccatcctccctccgtgatatatttatcagaa
atggatttgcttcacagataatcccagcaattgatactccatacgttgatattacgcactaaatttgacgctttattcttagggcctctttatgacgatt
atatgtgagatagctat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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