XB-CLONE-4354566
Clone Name: IMAGE:7026748
|
Gene:
stx11
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC081360
[+]
AGTTCATTTTGTTGGCCAGGAGTTGTTCGCAGCTGAACCTGATTGGGAAAATAAGCAATCATGAAGGACCGTCTTAATGAACTACTGGAATATGCAAAGC
TCCACACCCAGTATGTCCAGGGTGAAGAAGAGGATGAATTGCAAGGCTTTGGTGTATTTGACACTGACCATGCATTGGAAAGCCTGTACCAAGATATACA
GCTCATCCGGACTGAAAACCACTTAATGAAAGTTGATGTGAAGCGCTTGGGAAAGCAAACCACCCGCTTCCTCACTTCAATGCGCCGCCTCAGCAGTATC
AAAAGAGATTCCAACAGCATCGCTAAAGACATTAAGACAAGAGCTGAGGGCATACACAAAAAACTCCAAAGCCTTAAAAATTTAAGTGAGGATGCAGAGA
ACAAATCTGGAGAAAGTTCAGTCATGGCACGTGTATCAAAATCTCAGTACATGACCCTAACACTTGAGTTTCAGGAAGCCATGCTTGAGTACAATGAGGC
TGAGATGGTACAAAGGGAGAACTGCAAGATCCGCATCCAGCGCCAGCTAGAGATCATGGGAAAAGATGTGTCTGGTAATCAGATAGATGACATGATTGAG
CAAGGCAAATGGGATGTCTTCTCGGAAAACCTTCTGTCTGATGTTAAAGTTGCCCGTTCAGCTTTGAACGAGATTGAAACACGCCATAAGGAGCTTCTAA
AGCTAGAATCACGTATTCGGGAGGTTCATGACCTTTTTTTACAGATGGCAATCTTGGTGGAAGAACAAGCAGAGACTTTAAATGTTGTTGAGCTTAATAT
GGAAAAAGTGAAAGATTATGTGGGAGAAGCAAAAACTCAAGTTAGACAAGCTGTAGAATATAAAAGAAAGCACCCTTGTCGCCGCATGTGTTGTTGCTGC
TTTCCTTGCTGCAACTGATTCTTTTTCAAGTTCATATATTTTAGTACACTTCTCCTTTTGGAAAAAAATGTTGAAATTAACAAGCAGGGACAACTTGCGT
GGTTATTCATTCCTGGTTTTTAAGGTCTCCCACAGCAGTAAGAGGTATGTCCTCATAAAAAAGTAGGTGCATTGCAGTCTTAAGCTAAGGAACCCTGTAT
CTTATGCACGTATTAGTAAACCCCTTATTATTTAAATGACACAGAACTTATAAATATATCATATCTGCTTGAAATCATCTTGTCTTGGACCTTGACTATT
AGAAAATGAAATATTACAGTCAATTTTCAGAGAGTTTGCAGAGTTGCCACTTCGAGCCATATGCAAACCTAATGCTGTCTATGTATAGTATGAGTCTAGA
CAACTCACACACACCCAGAGCACATATATGTTTAGTCAGTATTATTGTATATGATAGTATGTATATGATCAAGAATAAACATATCATATCATGCTTAAAA
AAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF782913
[+]taattaatcccccccccccccagttcattttgttggccaggagttgttcgcagctgaacctgattgggaaaataagcaatcatgaaggaccgtcttaatg
aactactggaatatgcaaagctccacacccagtatgtccagggtgaagaagaggatgaattgcaaggctttggtgtatttgacactgaccatgcattgga
aagcctgtaccaagatatacagctcatccggactgaaaaccacttaatgaaagttgatgtgaagcgcttgggaaagcaaaccacccgcttcctcacttca
atgcgccgcctcagcagtatcaaaagagattccaacagcatcgctaaagacattaagacaagagctgagggcatacacaaaaaactccaaagcccttaaa
aatttaaattgaaggatgcaaaagaaccaaatcttgggaaaaaagttccagtcattgggcccgggggttaccaaaaaattcccagtaaaatgggcccccc
caaaacctttttgtgttttttttagagaaacaccccctcttttttttaaaacaaaagaaaagggggggggggggaaaaaaaaaaaaaaaaagataancn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: