XB-CLONE-4354558
Clone Name: IMAGE:7026744
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Gene:
tm9sf2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077062
[+]
GAGCAGGAAGGAGGAAGTTAGTGCGAGCTGTCGGTTGGAACTTAGAGCGGATACTACTTTGCTCATCTTATCGGGCCCGGGTTATATCAGTATCCCTCTG
CCGCACCGGCTACCGGGCATGGCCACCCAACCCAGCACTGTGATGTTCAGTTTATTGCTGCTGGCCGTTATCGGGCCCTGCGCGGCTTTCTACCTGCCTG
GTCTGGCCCCAGTAAGCTTCTGCGAGCAGGGAAAGGAAACAGCGGAATGCAAGTCTGAAATAGAGCTGTTTGTGAACAGACTGGACTCTGTGGAATCGGT
TCTTCCTTATGAATATGCTGCGTTTGATTTTTGCCAAAATACAGGAGAAAAACGTCCATCTGAAAATCTTGGTCAGGTGCTGTTTGGTGAAAGAATTGAA
CCCTCCCCATATAAGTTTATATTTAACAAGGAAGTAAAATGCCAGCTTGTTTGTAAAAAGACTTATTCTTCTTCTAACCAAGAAAGCAAGTCCAAACTTG
ATTTTCTGAAAAAAAGTATGCTTCTGAACTATCAGCATCATTGGATTGTTGACAATATGCCTGTAACGTGGTGTTATGATGTTGAAGACGGACAGAGATT
TTGCAATCCTGGATTTCCTATTGGTTGTTACATTACTCAAAATGGTCATCCGAAAGATGCATGTGTAATTAATTCAGAGTTCCACGAGAAAAACGCATAT
TATATCTTCAACCATGTGGATATCAAAATGTTCTACCATGTTGTAGAAAATGAGGGCCAAGGGGCAAGACTTGTAGCTGCTAAACTTGAACCTAAAAGCT
TCAAGCAGACACATGTTGACCAGCCAGACTGCTCAGGGCCTCCTATGGAAATCAAGAATGCTTTCGGTGGTGACGTCACTGTTCCATATACCTACTCTGT
AACTTTTCATGAAGAAAAAAAAAAAAAAAA
5' EST: CF782918
[+]taattaatcccccccccccccgagcaggaaggaggaagttagtgcgagctgtcggttggaacttagagcggatactactttgctcatcttatcgggcccg
ggttatatcagtatccctctgccgcaccggctaccgggcatggccacccaacccagcactgtgatgttcagtttattgctgctggccgttatcgggccct
gcgcggctttctacctgcctggtctggccccagtaagcttctgcgagcagggaaaggaaacagcggaatgcaagtctgaaatagagctgtttgtgaacag
actggactctgtggaatcggttcttccttatgaatatgctgcgtttgatttttgcgagatacgggagaaaacgtccatctgaaaatcttgttcaggtgct
gttgggtgaaaaaattgaaccctcccccatagaaggtttagttttgaacgaggaagtaaaatggcttgcttggttgtaaaaagaccttttaatccttctt
gctaaaacgaggaaaggcgtggtccctaactttggattgttctgaataaaaaagggatggctatgtggaaacttatccagccattcttttggggaattgt
ttgaacaaatacatgcccctgttatccgttggggtgtttatatcaaatgttttggtggaatatgggacccagaagaattttttgggaaaaattcccccgc
ggtaattttcccccatataatggaggtttgtgtttataaatattaacgcattaaaaaaaaattaagggggataacacttccgcgcgaaacaagaaagaat
ttatgcctggggtggggtggtgaaaaatttatagatatttttcgcagcgtgagttttattcctctcccctaatatttacaaagttcgtggtgtccctatt
gctcatttgangatgggggnaggcaaagataggtgttgagggtggagagatttcacgttacnaaagagattggatttcacatgtatattaacatctagag
cttgttttgcaggtaaaatatgggggggggggtactctaaatagagaactaaatcccaataaatttttttttaatacgctgtggagattaaaacattttg
gatgaatatctttctaagtgggtgttgtgatagagaataggaaaacatcgaaatggtaggggnaaactgagcgagtctagtaggaagggtgtgtcaaaag
gggagtgcttttctgtgtnggggggtcaaattttctctatgaaagttggtaatgtgccccgatagtaaaaaataaccacccccaggagcaatcctcaaaa
agcn
3' EST: CF782917
[+]tttttttttttttttcttcataaaaagttacagagtaggtatatggaacagtgacgtcaccaccgaaagcattcttgatttccataggaggccctgagca
gtctggctggtcaacatgtgtctgcttgaagcttttaggttcaagtttagcagctacaagtcttgccccttggccctcattttctacaacatggtagaac
attttgatatccacatggttgaagatataatatgcgtttttctcgtggaactctgaattaattacacatgcatctttcggatgaccattttgagtaatgt
aacaaccaataggaaatccaggattgcaaaatctctgtccgtcttcaacatcataacaccacgttacaggcatattgtcaacaatccaatgatgctgata
gttcagaagcatactttttttcagaaaatcaagtttggacttgctttcttggttagaagaagaataagtctttttacaaacaagctggcattttacttcc
ttgttaaatataaacttatatggggagggttcaattctttcaccaaacagcacctgaccaagattttcagatggacgtttttctcctgtattttggcaaa
aatcaaacgcagcatattcataaggaaaaaccgattccacaagaatccagtctgttcacaaacagctctatttcaaaattgcatttccgctgtttccctt
tccctgctcccaaaaaacttactgggggcccgaaccaggcagggtaaaaagcccccccaggcccccaaaaaacg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: