XB-CLONE-4354555
Clone Name: IMAGE:7026742
|
Gene:
hmgb3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782920
[+]
taattaatcccccccccccccggactccacacttagcgaacaatacagagtgaagatggctaaaggtgaccctaagaagccgaaaggcaagatgtctgcc
tatgcatattttgtgcagacatgccgtgaagaacacaaaaagaaaaatcctgaaatccctgtgaactttgcagagttttccaagaagtgctctgagagat
ggaagaccatgtcggctaaagagaaatcaaagtttgacgatctggcaaaggcagacaaagttcggtatgacagagaaatgaaagactttggacctgtaaa
gaaaggcaagaagaagaaagatccaaatgcccctaaaaggccaccatctggctttttcctcttctgctcagaattccgtccaaaaataaaatctacaaac
cctggtatttccattggtgatgtggcaaagaagctaggtgagatgtggaataaccttaacgatggtgagaagcagccatacaacaacaaggcagctaaac
tcaaggaaaaatatgaaaaggatgttgctgattacaaatctaaaggaaagttcgactgtgctaaaggagccccaaaattagcacggaaaaaagaggaaga
cgaggatgatgatgatgaagatgaggaggaggatgaagaggacgaagaagatgatgatgattaaagttgtacattctgtccctgtgaatacaatagagta
gggggaaccgaagtcaatgactccatcttaaataagggggtccactttgccctcatttaggttttattggcaaattttggtttatgggaatggtttccaa
aaggatgctccctggggaattggtaacctgggtttaactttaaagttaaccaagtgcggtggtttaaacatggaaccaatttatgaaaaactgggtcaaa
agaccttggacacttatttttccccaaaacattttttttttaaaaat
3' EST: CF782919
[+]tttttttttttttttgtcaagaacagtcttaaaaatgtaatactgttttatttttgcttcaaaaacatttcagcattctaaacataaaaaacataacaat
gcaaatataagtacagaacgtttttgaacttgcagatcctgtactcactcgctcttcacttcagtcagtaaagtagtgtctacagtctttgtttaaaaac
taccgcacttaactaaaaaataaacccataaatgtttctcatgccagctgaaccccttccgacaactaatggcagaatgctaatctcactatatacagaa
agacaactttaagctaaatagacgcccaatgtagtgtaagttgatttcatgtgcatgctttgggctagaaggacaaccccctttgaaaagggtgacctag
tccagggaagtttgtaaaccctgaggtaccaagagctccaagaaatatgcaccataggaatttgtctagtttacagattacagatatgtacaagtcataa
cccaggattttctaatcaataaccattaagtaacaccttaaataaaaaaatgcttgaaacatttttaaatgctttgttacactaacagcaaagtgcacag
agtgaggagaacactagagcgctttttttctttaaaaaatgtttggaaatatgtacagctttgacagtttcatattgctcatgttaacacgcactgctac
ttaatggaaaccagttacaatcccaagagcatcattatgaaaaattccataaccaaattttgcaaattaaacctaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: