XB-CLONE-4354553
Clone Name: IMAGE:7026741
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Gene:
cdca3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF722992
[+]
taattaatcccccccccccccgattgtgcagaagggaacgatcgctttggagaccgtacaccccccacttgtcgctatgggagcctcagagtccaaagcc
caggtaaccccttcccgtcctctgcgtaatcacctgctgtctcgtgtgaatgatccccgctccccaaccagcgggattcccaggactcctattgaggtgg
gggagtctccccggaacacccctcagactgctcacgaggaggaggaggagatatttgaggaggagattcttgatactccagagatctcagaccctcgttc
cccaaccaatggtatcacacgcacccccctcagaccccccatgcatgctgttctgaacaacctggcaaagcagttaagtgaagtgtttgtggcagaggat
tctgtggctgaaggcggcccgttgagccccactggtcatgaagctgcttatcttgagaggcaagtaggaaaggagtcccaaatgagtcaggaggcatgtg
tgggggagaagaatgtgaatggtggaaaaagtggattcggtggcagaaatgggcaaaaacttccgaatgctccaaaaggtgggcctcagaagttaaaaaa
gggggggggactcccaaattgattcaggagggagagttctgaaatagctgaaaaaaggccaaaaactggggtaagtgataagggaatcccatttccgtgg
gggcccaagactgggggatatcccaccggaaaccttataccggggttttggggaaccaccgagcggaaggaatcggagggaaaatccccccccgtttccc
ccgggggggaaaaaagaaaattcccattcaaggtgacccaaaaaaatatctttgggtgtggccgtggaccatttggggccggattttttttttccgggga
ttctgaaaatgaggggttaacactctgcccaacat
3' EST: CF782921
[+]tttttttttttttttaatgacaaacaagtaaaattctatttattaatgtcacatagagagaactgcacagatacaaacacacagaatatttacacaaatt
gtgtaagaatttacagatgtaacttctttacacccaggagaggggccacagcagcaagtccttaataatggccaagtaacggcaccgtttcatcagaaat
atagggtttgtcacaaggattcccttgcctttagtaagcagggataatagtgagctcagcgcactcctctgtacagctgcctgtctccttatacacataa
agcagctggaattaataagtagcagattatttcccctttagaatcactcagctggtgctgtgcccatactcagcgttctctttgttgagcgatgactccc
agttaccgtgtgagattttaagcgctcgatggggcagcgcaagctccgtctgggaagaacacttcttacccggtcggtgttgccgcaaaagggttgggcg
aatttaccctcctggagggatccggaagaggaaatcggcccttatgacgagggacaggcaaaaattttcctgggtcccctgaaggacatttctttatccc
ccgaaggaagcgggaaaattttccccccgaatccccgggcaggggggctcaaaaccctggtaataggtgtcgggggaaccccccaggatggggccccccc
gaaaagagaattccggaaacaatacaaacttcttctgcccttctccccgtataaaaaaaattctccctcccgggtaaaattttgggaatctccccacctt
ttttttttttttttcggaaaaaccccctccttggagggttttttgtagtttn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: