XB-CLONE-4354550
Clone Name: IMAGE:7026740
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782923 [+]
taattaatcccccccccccccccttccttttacattcttttatttctaataaaactgttaaattaaaaaaaataaaaaaaaaaaaaaaaaaaaaaaaaat
ataaaaaaaaaaaaaaaaaattaaaaaaaaaaaaaaaggggcccaatttctaaaggggggcgacaacaggggggaaatgggggggggccaaaaatttttt
tttgaaatttaaaggggtaaaaaaaaaacattaacatccaaaattttgaaaaaaaaacttttttttaacaggaataaagattgggatggtacaaactcca
caagggtaataaataaggtaagggttagggaaaaaatattggggcacaacaagggaaagaaaaaattttaaaaaaaaaaattggttaatgaacatataaa
gcaaaaaaaaaaaaaccttggaatatattataataatagaagatgaataaaaatacaaataatcctcaaaaagaaaaaaagatatataataaaaagagag
caaataaaaaaaaattgcaaaaaacctatagtaggggaaaaaaattcacccaagtgctttcttatgagtgtggagatataagtatgtgcataagacaaat
taaagtttaataaaatattaaagaaaaaagataactaaaaagatgttcagcaacacccataaaaatctagcagtgcaaccgtggcacggccgacgcaata
taaagaatataaacgagtagtatataagngttgagcaatagaggcatatatcactgtaatgaacccatcacgctagagaggtgaatagaaaagtgacata
aaatagttactatttacatgtgcgagtgactttttgagtaaangcaataaggaatactcgagggagggttagtttagaaagaggagaccgtaagnganta
tncnaataccggaccacccgccgcacctacagtgcaaatacagtaggagcgacatggtacttattacgtgcagagagcgtgaagtgtatgagtgctctac
tnaaaagagggaactaggatgaacagnanacaaaattattatgttgtaggcagggagaagccgtcagaacacnaaaaaacaaagaattaataacttaacc
actaaaattaagaagaaataatatagaattgggggaggataataacaaaaatatatttattattgtatcttcccagaacaaaatctttttatnataaacc
aacaatatcntgtggtaacttattgttcatcacnaaatatatatcaacatatgaaacaaatattaataaattttattccattattctggangtacataag
ncgacgcgttagtgataagtaagtaatgataatagtttaacacgaaaagaagaaaaatatatttcataatacataattaaaataaaaaacacaaccaaaa
gaaaacagcgcacctaggagaagcgagcgcgaagtgaaagggtgaggagttggtcaagggatggtnggaagagtcaataggtgtagaggccaggacgaga
atttaaacaaaattataaaatgttatcttaatatattaataatatattcatgcgn
3' EST: CF782922 [+]tttttttttttttttattttttttttttttttttttttttttttttttttttttttttttttttttttttttaaattaacccgtttttttaaaaaaaaaa
aaatttaaaaggaaggggggggggggggaattatttaaccccattgcaaaaaaaaaaatttcttttttttccgccccgaaaaaaaccaaggtccatttga
ccctggaattgaacccctttgaaaaaaaaggcaaagggaatttttttctaaagggcccccaaaacccaatttttaaaaaacaaaaggtttttttttattt
ttacccccttttaaaaaaacccaaggggggggccctaaaaaccctaaagggggatttttctccccccttggcccgggaaaccccctttcaaatgcccctt
tcccccccccaaaacctgaatcccccccggttttttctttgggggccaaaaaaccgggaatggcttcccccggcaatttaaggtttccataaaaaacccc
tctttttaaaaccccccccccaaaaaccccaacccccctttttctcaaatggggcaaaaatttttccaaatttttgaaaacccccctttaattttggggc
ccaaaaaaaaaccccctttttccctacaagggggggaaaatttggaaaaccccccctgaaaacaaaaccctttttccccccccccttttttttttttgtc
caaaacccccttcccctttggtaaaaaccccataaaaataaaaccctaaaattttctcccccacaaaaaaagaaaaccccccataaaggcccttgttctt
ggcccaataattcccccgggggggccatttttaccacccttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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