XB-CLONE-4354527
Clone Name: IMAGE:7026730
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Gene:
cd63
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782937
[+]
taattaatcccccccccccccgagagtcactcgccgcaagttaccagggaggagaacgagaaaggcagagcggcaggatggcggtggaagggggaatgaa
atgtgtcaagttccttatgttcttcttcaacttcgtgttctgggtgtgtggcattgctctcattgcgatcgggatatacgtgcaaatccagcttaaccac
actcttatcatgaagaatgccacctcctctggagctcccatagtcatcatcgtagtgggtgttgtcatctttttaattgccttctttggctgctgtggag
cactaaaagagaactactgcatggtgacaacgtttgccgttgtgctggtgctgatcttcctggttgagattgctgctgctattgctgcctatgtttacaa
ggacaagctgcgcactgcatttgaggactcctttaaaaatggtatggtccattataatgacaccaaggatatagcggatagcattgacctcttgcagaaa
gaatttcagtgctgtggtgccatcaactcaactgattggagacaatatgctcccttcactggtacaaacactgtgcctgattcctgctgtaagaacatca
ctgcaggctgtggaaaaggtccaattgccaatataaatagtgagggctgcgcaaccggaatagaccagtggggtgaagaaaaatgtangaattgttgctg
ggagtggnccctgggaattgctttgttttgagatcttgggggatcgtcttttgcctgctgnttaaatgaagggaatcccgaagtgggatacgaagttcat
ggtgatcgcctccaccagtgggataatgccggtggatgtcacctttgtctttgcatttaaccagtttggggaaatgggtattgcacccatctccacaacc
aattttggcaat
3' EST: CF782936
[+]tttttttttttttttggtatcaaagtatttatattaagtacaaagagcatgtcattattccccacgttaaggtgggaagtggtatgtgccatttattgcc
aaattgctgtgagatggtgcataccacttcccaacttgctaatgcaagacaagtgacatcacggcattatccactgtggagcgatcacatgacttcgtat
ccacttcggattcccttcattaaacagcaggcaaagacgatccccaagatctcaaacaaagcaattcccagggccactccagcaacaattcctacatttt
tcttcacccactggtctattccggttgcgcagccctcactatttatattggcaattggaccttttccacagcctgcagtgatgttcttacagcaggaatc
aggcacagtgtttgtaccagtgaaaggagcatattgtctcccaatcagtgaagtgattgcaccacagcactgaaaatcctttctgcaagaggtcaatgct
atcccgcaaaatccctgggggtcattaaaaagggaacataccattttttaaaggagtccctcaaaggcaatggcccaccttggcccttgtaaaactttgg
gccgcaaatggcagcccgcaatcccaacccgggaaaaattagcccccgggccaaaaggggaaaagtttggtccccctgccaaaaaattccccttttaaag
gggccccccgggggccaaagaaaggggcttttaaaaaaaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: