XB-CLONE-4354521
Clone Name: IMAGE:7026727
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Gene:
selenof
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF722999
[+]
taattaatccccccccccccccccgagtgcctagtgggaagatggcggcagagaggatgctgctgtggttagtgactgtgctacaggcgctgtcgtctta
tggtgcagaactctcttcagaagcatgcagagatcttggcttctctagcaatcttttgtgcacttcttgtgaattgcttggacagtttggtctgaatgag
attttttttttctgcaggcagtgctgccaggaagaagtacacctagagtctaaaaagagatatccaggtgctgtacttgaagtctgtggatgaaaattgg
gaaggtttcctcaagtccaggcttttgtcaggagtgagaaacccaaacttttcaagggtctgcagatcaagtatgtacgtggctctgaccctgtcctaaa
gcttctggatgaaaatgggaacatatctgaagaactgagcatactgaaatggaatacagattgtgtggaggaatttttaagtgaaaaactagatagatta
taaagagtttttttctattttttagcctttaatttgaaaagtttcatggacaaatttctgctttctgagggcataacatcctagcattatttttgcattt
tataaaatttctttgggcaggcaattccatttttttaaagtggcataaccccctcctggaagtgggccttaacccaagggggtaaaagaatttttttagt
tggtcagcttgttccctggggttactggaaaaaaatgggttgtttttggcctattgtgggaaataaggataaacaggcttcttttttcccaggaggggga
aaggtttttttttttgcaatcttatataacnttttttttatatattttctgaaataaaacgatttttgttagagaacaagccccccaaagtgaatttcac
gtggggttataaataaattggggttctgaggaaatgaagtggaggggagtctcccccccctacctattccgtttatgtaaagcccattgagatatgtaat
cactttttgtaacaaagggggcgtgggaagaatgttttgc
3' EST: CF782940
[+]gcgtttgcaagacacatttagaaaaatccagaaatacaaaaatgcaaatggaagcatttatgacaccaaaagacagggtaaaattcaaataagttttttt
tttttttttgaaagaaagaatccaatcaaatctttggctttagacccagtatacagccctcctggccccccttccaactgcagctatagcagctaccatg
ttggtgaaagcagcgagtgcagcaactaggaatggggacactgatccatcttagccacgagtgtgcatacaaattgggtgagcaaacattgtgcacacag
tattttgagtgaaaataatttagtacttattgtgagtagcccacttctcagcacaaactgctctgtttttaaagcacacaaaaatagcaaaaaggaaatt
cgaggaaatagtgattaaggaatgttcactgggactagaggcaaaagcttcagtggtgtttgctttccctttaaacacatttaaggcttctaaaattgta
cagaagtgtaacaaagagaaaaagtctgtttataaacaatgttgaaataacttactgaatccaccctgtgtttatggtatggtgcacggaaaacttcatt
tctgaaatgcacaatataaatgataatctaatggcaatggtgcaagtttacagactacgtttggcatcctcatcctaaaattgcccaacatattttgtta
tacatgtcaaaaagttttattttacaagtgagggccaaaaagttgtgccacattaaatttttgtccagcctaaaggtttctagccttccctttaaataag
gt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: