XB-CLONE-4354511
Clone Name: IMAGE:7026723
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Gene:
aldh18a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723000
[+]
taattaatcccccccccccccgacggcgattccagaaacaatattgtgagctgcgtttctttgcactgaaggaaagatccgtaaatagcgcagcgttgtg
gttctgaacgcggtgtcagggatacgtcactgtgtgtgtgcggaagggctgcgcaaggagcctagtggggctttgagaatagccggagcggagagcaggc
tggatagagtacgaaaatgttgcatcgggctgctctgagttcctgcgtgggcacctctgccagatgcctccttgcacgggtacgaagcacggcttcttcc
aacttgttacatttccctggctctactcatcagacagcccgtcattggagcaatatccctttcatcacggtgccacatatgcgctctcatgggaagccat
ttgcccaccgcagtgagctaaagcatgccaagcgaatcgtcgtgaagctgggaagcgcagtagtgacaagaggggatgagtgcggcttanctcttgggag
gctggcttctattgtagaacaggtgtctgtgctgcagaaccagggcagagagatgatgatcgttaccagtggtgctgtggcatttggaaagcaacgcctg
cgccatgaaatcctcttgtcacagagtgtgcggccaagccttgccactcagggcaagaaccagcctggaaagatatggcaaagttccaattgtttgggaa
accccggtgcctttgttgccccccggcccgggccca
3' EST: CF782946
[+]ggctgtgctgattcccacctccgctccaagtccaaatcggtatccatcagaaaatcgagtgctggtattccagaacacacaagcgctatccacgtgctgc
agaaaatactccgctgtttgttcattctctgtgataatagcatccgtgtgggaacttccatacttgtgaatatgctcaattgcatcctggactccatcta
ccacttcaaggcaacactccaagtcaccatattctgtacgcagagatttcacttctgaggggctgaaggtcaggtaggaggcaaaccttggaccggcatg
gattttaacctgttcaaccctcagcatgtcaatgatctggtcaaacagtggggttctcagtatatctctatggatgagcagtgtttccattgcattgcaa
gcagctggatattcacacttggagtcctgcacgattcttgtgaccttgtcaacacttgcatcagaatccacatacacgtggcatattccttcactgtgcc
ccatcacgggaatacccttggctgctttctgaatgtccctgaccaattgtgaggaaccacgtgggtatgatcagatcaataagtttgtttaaacgacaga
ggtcttcaacttcttctcgggtgttcaccaactgcacaaggtccctaactccattgaaaaaacatgcttccctgaattcagttcggaaaagtaatctggg
tgggtgttggccggcccccctttcccccttttcagaaaccaaaccttttcccacctaaccaagggcccatggcaaaaaacctgtaggaaagggcatcttg
ggttttgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: