XB-CLONE-4354506
Clone Name: IMAGE:7026720
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Gene:
sec61a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782949 [+]
taattaatcccccccccccccccgagtctaaggagagaccgcggcgcaacaggaccggtcccccagcccgtcacacccctagaggccactgagcccaccg
caacaccttcaccatgggcattaaatttcttgaagtcatcaagccattctgtgcaatactaccagaaatccaaaagccagaaagaaagatccaatttaag
gaaaaagtactatggactgccatcactttgttcatcttcttagtatgttgccagatcccactctttggtattatgtcctcggattccgctgatcctttct
actggatgagagtcattttggcttccaacagaggtacccttatggagctcggtatctctcccattgtcacctctggtctaatcatgcagcttctagctgg
tgccaagattattgaggttggagacactcctaaggaccgcgctctcttcaatggtgctcaaaaattatttggaatgatcatcaccatcgggcaagccatt
gtgtatgtcatgactggaatgtatggggggatccttcaaaaaaggggggctggcatctgtcttcttatcaccatccagctttttgtttgctgggcctgat
atgtctggctcctgggaagaaatttgctaaacaaaagggctatgggttaggggcccccggggaaacctctccacctttcatgggctaagcaaaaaaatct
gcaaaaaacccatttttttggaaaaggcaatttaattcccaacctacctttggaaacaccaaggcagaagggacccctaagttttagaggggaaccagat
cacttgcgcccggattacacattggatggcccccgccgtactaggaaaaaatttcccgcccccttagagagggaaggcctttttcttccagcacaagaat
gagggccgaatcctcagaaaaaaccgtcatagagctgcacatttgttcgcgttttttggcggggagacccaaaaaccctctccggggg
3' EST: CF782948 [+]gcatgaagactatgtaaaccactgcatgaaccggatcgtccagtaccgaaccaaatgattccggcggtgagaggtaataacataacccgcctacaggata
ggcccgagcaggacctccagtcgttgcatcagaccatgtgcccaataagttgacaaggaggttgccactgaaacgggcagacagcatctgggagataacg
tacagatttgagaccagggcagactggagaatgatggggatgttagaagtatagaaaagcttgattgggtaagtgttgtactggccacggtaccgggctg
acttgattggaagatccactctaaagccctggaagtatatgactactgcaaaaacgaaaatggtagcgatgaggttcatgagattgggcaggttctggcg
atagaaggcctccctcagagcgcggactttgtcagtacgtgtggcaagcaagtgaaacagggcaatgattgctccctcaaactcagtgcctctgcctgtg
ttccacgtatttggactgaatgccttccatacaatggtttcgcaaatgtttgtagccatgaaaagaaaaataccgagccccaaacatagccctttctgta
gccattccatccagggaaccaaaaaaatcaagcccgcccaccaaaaaagctggaaggggtataagaaaaaaagatgccccccccctcatgttctgaaggg
atcccgcatacctttccctgtccttgacaatccaccaaggggtttgaaccgaaagggggaatgaaccatatccgcaattaatttctcgagacccaccttt
gaaaaaaaaaccccggtgtcattaatgaaaatgat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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