XB-CLONE-4354503
Clone Name: IMAGE:7026719
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Gene:
ocm4.4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782951 [+]
taattaatcccccccccccccggcaagcctcattgagaagatctgtaaagtcccgtgaaagaggaattaaggacacaaaatggccttaggtgctctcctg
agtcaagctgacatctctgctgctctgcaggcttgccaagcccctgactccttcaactacaaatccttctttgcccagtctggcctgagcagcaagtccg
tcgatgatgtgagaaacgcctttgccatccttgaccaggacaagagcggcttcattgaggaagatgaattgaaactgttcctccaaaacttcagcgcagg
tgccagggctttgactgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgaattccaggctttggta
aaggcttagacgttcaatcacggtgagtcttgtgaaactttgctaagcgtttaacccatcccacgaggtgttgatttacttcgaaatgagtagcagggga
agaaactgaagcatcgtgggttttgatgggagagactaaagaatttttcatttggcatgtggtttttctattccgtggaggggaacaaagaccttttggg
gaataacatataaatgtgggacatttgtgacagaataatcttcacagaggtttatatttataaattgacttgttccgaggttggtaataccagcaacata
aatgttgcctngtgtaaacaaccaaattctataagcttgnacccacaatgatattgagggcaaatctccccctcgtggactggggagcacccgcgacaac
ccctgataattatgtggtgcctttttccccttgttgccatgggtgggattttgtgcgctgggctnccgtgtgagagtataaaggggagaaggngatatgt
gagaggcaagctcnataggcgtgtggtgaaatgcggtaantattatgagaagaaagtaataatttgtggggggtgtaaagatggagatgtatatataaag
aaagaagaatatcaaaatacaaaccaacaaattaaaacatatatcactgaaagtagtaattattggagagtaaaagagcgacgaacnaaagataagagat
gaggggaaagagaaagacaggatatattgaaaagcctatccgggattgggggtccggcggatgggcggtggggcggtgtcgtgggcagtgacggggccgg
cgggaatatctgcgtatggctaggtagtgccgaagtggggggggatggcgaggctatataaatcggctcgaaagagaagaaaaatggtgatagtgaagcg
gttagataatatagatggtgctaataccaggatatagtaaatctatatactttctgttatgtttctcatacaaaa
3' EST: CF782950 [+]tttttttttttttttcgttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttttttttttttttttttttttttttttttaaaaaaaaaaacaaaaattttttttttttggggggggccccccccaaaaaaaaaaaaaaaa
aaaaaattgggggggggggaaaaaaaaaaaagaggggggaaaaaaacccccccccccaaaaaaaaaaaaaaaaaaattttttttaaaaaaaaaaaaccgg
ggggggggggggggaaaaaaacaggggggggggggggaaaaaaaaaaaccctcctcaaaaaaaaaaaaacccgggccccccccgggggggggggatcaaa
aaactccccccccgaaaaaaaaaaaaaaaaaaagggggggggggacaaaaaaaaaaaaaccttttttttttcttaaaaaaaaaaaaaaaaaaaagggggg
ggggggggggggtaaaaaaaccccccccccccccggggagaaaggagacccccccccaccccgcgggggaacccccccccccttttggggcccccccaaa
aaaaacccaccacgaggggggggggggcgggggccccacacacaaaacacaccccccccccccccccccaaataagggggggaagaaaattttttttttt
tccccccaaaaaaaaccccccccctctaaatttgaaaagagagagaaaaaaaggaggggttattccccaacccaaacggcggagggcttgttcccccccg
ccgcccgacggggagagataaaaaaaaagagatttttactcaaaacaaaaaaccaaccgggcggggcggttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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