XB-CLONE-4354501
Clone Name: IMAGE:7026718
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Gene:
calml4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084515
[+]
GAGAGTTAATCCTACAGTTGCCCATTACAAGAAAGGATTCAGCTCATCTCCTCTCTCTGTGAAGCAGCTGGGAGGCTCCATTACCTTTACACCATAAATA
TTTAATGGGCAGGAAGGTGTAAGATAACAGCAGTTAAAGGACAGTAAGAGGAACCTGCCGTTCCTGTGACTGGGATAGGGAGCTTGAATCCAACCCCGAG
CTGCCATAGCAGCAGGATATTTGCTTCTCAGTGTTTCACATTATTACTGCGTGGACTTTGCCTCCTGCAGAAATCAGGAAACAGCAGCTCACTAGAACGA
GATGGCCAAGTTCCTGTCTCAGGATGCAATTCAAAAGTTCAAGGAATGTTTTTCTCTCTATGATAAGAAAGGCAAAGGTAAAATCCCAGCCGGGGATCTC
CTGACTGTCATGCGATGCCTCGGCACCTGTCCCACGCCGGGGGAGGTCACACGACACCTGCAGGTCCACAAAATCGGCAAGGATGGGGAAGTGGATTTCT
CTACCTTCTTGACAATCATGTACCGCCAGCAGAAGCAGGAGGACCCAGAGAATGAGATCATGGTGGCCATGTTAATGTCAGACAAGCAGAAGAAAGGAGT
CATCCCACTGAAAGAGCTCAGAGCCAAACTCACTCAAATGGGGGAGAAGCTCACACCAGAGGAAGTTGATGATCTCCTAAAAGGAGTTAAAGTGGGACCA
GATGGGATGGTGAAGTATGAAGAGTTTGTAAGGCAGATCACACTCCCTGTGCCTGACTACTGAACTGCAACAACGCTGAAGATCCGAAATGTGTGTGGGA
CCAGTTTTAGATCACCATGTGTTGTTGAGTCCTGCACTCAGCTTCCTGAATGGATGTTAAAATAGCACTCCCAGCTTCCTCAGCCACCCAAGGCTGCACC
TATCAATACAATCTCAAATAATACCACTTTACTTTCTCAGCTCAACTGTTATACTCACCATCATTTTCCCACTGTTGCCAGTAAAACACCAATTGTCATC
ACTGAGCTGACAGCTCTAGCCCTGCCTTTTAGTTCTGTCTACTGGATAGATCTTATAGAAGAGGGGAATTCAGCGCCTTATAATAAAGTAATTACCAGAA
AAAGTGCCATTTGTGTATTTAATATAAATCTTTAATATAGAATCTGTACAGGTAATTATCTCTAAGCTAAACAGGTGTAATATATGCCACCAATAAATGA
GAGCTGTTAGTGCAGAAAAAAAAAAAAAAAA
5' EST: CF782952
[+]taattaatcccccccccccccgagagttaatcctacagttgcccattacaagaaaggattcagctcatctcctctctctgtgaagcagctgggaggctcc
attacctttacaccataaatatttaatgggcaggaaggtgtaagataacagcagttaaaggacagtaagaggaacctgccgttcctgtgactgggatagg
gagcttgaatccaaccccgagctgccatagcagcaggatatttgcttctcagtgtttcacattattactgcgtggactttgcctcctgcagaaatcagga
aacagcagctcactagaacgagatggccaagttcctgtctcaggatgcaattcaaaagttcaaggaatgtttttctctctatgataagaaaggcaaaggt
aaaatcccagccggggatctcctgactgtcatgcgatgcctcggcacctgtcccacgccgggggaggtcacacgacacctgcaggtccacaaaatcggca
aggatggggaagtggatttctctaccttcttgacaatcatgtaccgcccgcagaagcaggaggaccccgaaaaatgagatcatgggtggccattgttaat
gtccagaaaagccaaaagaaaaggagttcatcccaattgtaaagaacttattaaaccaaaactcacctccaaatgggggggaaagaaacctcaccaccaa
aaagaaaatttgaatgaatcccccctatacaaggaagtttaaaagtggggaaacacaaattggggaatgggtggatagtaatgaaanagattttttgaat
gggcagaaattacaactctcccttgttgcccttgaccattctggaaacttgggaaacaataagcgattaaaaacataaccgaaaaaatattgtagggggg
ggaaaacctcgatttgtaagtatactacaatctaggagggtcttgtgtgttgaaagatacttggggaatgcgttgataatttttccctnctaaaaatggg
gatgttggttattataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: