XB-CLONE-4354498
Clone Name: IMAGE:7026717
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Gene:
des.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782954
[+]
aaggttttctggggaagccgctgggtaccaggactccattggacgtttggaggaagagatccgtaatatgaaggatgaaatggcaaggcatctccgcgag
tatcaggatctcctgaacgtcaagatggcgctggatgtggaaattgccacataccgtaaactgctcgagggcgaggagagcaggatcactcttcccatcc
aaacatactctgcactcagtttccgagaaaccagcccagagcagagagcttcagaagtccacacaaagaaaacagtcatgatcaaaaccattgagacccg
ggatggtgaggttgtaagtgaggcttcccagcagcaccaggagatcctgtaagccgccatcttgtctccctggccgtgggcacgcaacaggaagaaaaga
acacccacatggcacagcctgagtgcaaccctgctggacaaatggcatctcatgttgtaccttcaccacattaccttctgcacctatcacttgtcccttc
tgtctaaggaccccttgtccccaaagctcatataggagccgctcagtattatgaactctcctgctaagagacatccgtgttggggatgtctcggcagaaa
tacagggaaaccggaaataaagtcacagtcacccatacaaagccttgcagtccctattcattctcttagatacactctaatgaaaggcgcaatatagtaa
taatttagtaataaacacacaattacactgtatcctgccctggtaatagagcaaatcttttcatattatatgcccaacctcatccatggaatccatgttt
tccacaaggtcccattgatgaatggtttctaattaacagccccctaacaaataaatatatatac
3' EST: CF782953
[+]tttttttttttttttgtatcttaacaggtttattaaggtaaaatttatcacaaatgttctacgtatccttcccttactctgtgtgtgcggatagagtcct
acataagtcgtttgtggaattaacaaaaataaaagcccctagagctatcgtttgatagacagcttgtcggttccaggagaaaccttcattttctatatta
aaaatagaaaatctgatcttgtgctgtatccccccccccccactaggaatttaatgggattgtgctgggaattccgtagccgcaaggttattgaaccagt
ggctttctccttcgttcctacccccttattagtgataataacatctgccttccttcagaaatacccaataaaagacaaaacaaagttctatgcaagatcc
agagctgcagatacataacggaacccttcctctcccctcagacccccccccccgcgtatgtttcccgttcgttagcgtttccttcttatctcgtttattt
gtgacatgcttttccacttatttttttttaatcttcctggatttccagaagcttcagcgaaccctcagtgaagtgcattctgggtagaatcgtgcagcaa
attccaaatggaatgacctgtgtggggctagtggggggcttcctaggatttaacatggccagcgagtagaatttttatcaggcttgggagacatttgtct
ctctttacggcatctgcaacctgggttacttcacctcttttctgaactaaatttcccatcagacctctgtttcctggatctaaataaaaggcagccccat
tgtgat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: