XB-CLONE-4354490
Clone Name: IMAGE:7026713
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Gene:
ocm4.4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782959 [+]
taattaatcccccccccccccgaagcctcattgagaagatctgtaaagtcccgtgaaagaggaattaaggacacaaaatggccttaggtgctctcctgag
tcaagctgacatctctgctgctctgcaggcttgccaagcccctgactccttcaactacaaatccttctttgcccagtctggcctgagcagcaagtccgtc
gatgatgtgagaaacgcctttgccatccttgaccaggacaagagcggcttcattgaggaagatgaattgaaactgttcctccagaacttcagcgcaggtg
ccagggctttgactgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgaattccaggctttggtaaa
ggcttaaacattctaccacgtgagtctgtgaactttactaaacattaaccatccacaattttgactttttctaattaaactgaaataaacggaaatctgg
ttttgtggaacaccaccatctcttcgctgtgtttctatccgggggacaagttttggaaacatcacctgcttgcggaatccaaggattttattctgcaaag
aagacattttcgttaccatcaaccaacaatggatcctatgaccagcaatgcggcctaagattcatctaaaaaaagataactttcttaatttttgtaaatt
tggtaagagcatttgatcttcagcctgtcaatgaactttttggaaaattcccccagacaagaaaattccttttctttatccaatggtccttaaatgggga
tttggaagcccggccagaaaattaaagagaaaaaacctttgggggggcctaatccaaataaaaatcctggtttttttgggaaaatcccccccaaaaaata
ataaaaaaaaaaaaaaggggaatcccccccccttaaatttttctttaaagagagacgcga
3' EST: CF782958 [+]tttttttttttttttgtgattccaaaacagatttattgatagcacacaagtttttctttattctgctgcttcaatccattaagacattgaaagaaagaat
tctgtttgtggatttacaaaagtcatgacagctgagatcaatgctctaccaatatacaaaaataagaagttatctttattagatgaatcttaggcgcatt
gctggtcataggatccattgttggttgatggtaacgaaaatgtcttctttgcagaataaaatccttggattccgcaagcaggtgatgtttccaaaacttg
tcccccggatagaaacacagcgaagagatggtggtgttccacaaaaccagatttccgtttatttcagtttaattagaaaaagtcaaaattgtggatggtt
aatgtttagtaaagttcacagactcacgtggtagaatgtttaagcctttaccaaagcctggaattcatcaactccaattttgccatcaccatcagagtca
ccagcattcaggaaggccttggtctcagcatcagtcaaagccctggcacctgcgctgaagttctggaggaacagtttcaattcatcttcctcaatgaagc
cgctcttgtcctggtcaaggatggcaaaggcgtttctcacatcatcgacggacttgctgctcaagccagactgggcaaagaaggatttgtagttggaaga
gtcanggggcttggcaagccttgcagagccagcagagatgttcagctttgactcaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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