XB-CLONE-4354484
Clone Name: IMAGE:7026710
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Gene:
lsm12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782963
[+]
taattaatccccccccccccccgacacactcttgactgcctctgttaggaactgtgacgcttgtgagggctgcctctctgatcataccacaaaatgtctg
acaaaccagatatggctgagattgagaaatttgataagaccaagctgaagaagacagaaacacaagaaaagaacccactcccatcaaaagaaacgattga
gcaagagaaagcaacttcataatgatcccccatcacccagatatgcactgtacattccacaagcattgccttcttattttacttttagctgtttaacttt
gtaagaaataactcaaaatacaaagaatttggagaacatgttctgtgctgccctgttacaccaaaagaaaggaataagaactactgaacacggaatgaag
gcgctgcctttccatctgcctgtctggctggctttggttctggtggcattganagatctttcttgattatttaaggagaaccagtgtggaaataacagaa
ctaaagtaacatgctctgatgtgggtgggatgaaagctgcaccaaggtccagcgagctgtaaaataccgtcaaatcgagtgccattttatttgttcaaat
taattgaattattggaatgcacaaatttttttttatattgcaataaaaatggataaaccttttaaaaaaaaaaaatagaaaaaaaaaaaaaaaaggatcc
cctattttcttaaagcggccgcccaccccggtggaaactttggccgtggccccacctttgtttattggcagcttaataaagggtttacaaaaataaacca
atatgcattcccaaatttctcccaaaataaaacgccttttttttttccccggcattttcaaaattggggggttgggtcccacaccccccacacaggggat
atcttatattcaatcgcccgggggattctggggactantatatattctcgcggcccccag
3' EST: CF782962
[+]tttttttttttttttctttttttttttttttaatgttttatcatttttatttgcatattaaaaaaaatttgtgcattccaataattcaattaattttgaa
caaataaaatggcactcgatttgaccgtattttacagctcgctggaccttggtgcagctttcatcccacccacatcagagcatgttactttagttctgtt
atttccacactggttctccttaaataatcaagaaagatctttcaatgccaccagaaccaaagccagccagacaggcagatggaaaggcagcgccttcatt
ccgtgttcagtagttcttattcctttcttttggtgtaacagggcagcacagaacatgttctccaaattctttgtattttgagttatttcttacaaagtta
aacagctaaaagtaaaataagaaggcaatgcttgtggaatgtacagtgcatatctgggtgatgggggatcattatgaagttgctttctcttgctcaatcg
tttcttttgatgggaagtgggttcttttcttgtgtttctgtcttcttcagccttggtcttatcaaatttctcaatctcagccatatctggtttgtcaaac
attttgtgggtatgatcagagaggcagccctcacaagcgtcacagtttcctaacaaaggcagtcaaaaaatgtgtccggggggggggggggggattaatt
aacctcgagggccaaaaaaaaaaaattactttgtaattccgggccccggaaaaaaaccccaaggggccatttggaccctgggaaggggaacaccctgtgg
aaagaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: