XB-CLONE-4354479
Clone Name: IMAGE:7026708
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782966
[+]
taattaatcccccccccccccgccttttctttccccaccctccgccggcactgatcctccctgtgtgctccggcactacaacacccaggccgccaacatg
atcatctacaaggacaccgtcactgaggatgaaatgttctccgatatctacaagatcgccgaaacccccgacggcatgtgttttgaagtggaagggaaga
tcatccaaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctgagtgccaggaggaggacatcggtggtgcaacaacagtcag
cggggtggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactcttacaagaattacattaaggattatgtaaaactagtcaaggcc
aaacttgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagatagagtaaaactcatacttggaaacttcaaaaactatcagtttt
acactggagaaagaatgaacccagatggtatggggggctctgctaaattaccgtgaggatggtgtaacacccttttatgattttccttaaagatggcttg
atcagtgaaaaatgttaaaatttcaattttggttcctcccttcttggctgctgccttttctcccatgaacaccaggtcttaagcaaggattttaccggac
tccctaagggactgatggatcctttgaggctggttttcttcctgggcccttaaattatttcaagtgggaaggctttttttggtttttttaaattgaaaac
cctttttgctatttaaaaatgaaaaaaaagccccaatattttctccccctggtaaacaattgcctcccttggttttgatcttccaatttaactttttgaa
aacaagcgggtccttgggggatttaaaagaatttgg
3' EST: CF782965
[+]tttttttttttttttacattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgttaacaggcaa
cagtcaatgttccaaatccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccagaggtatttgcaacactgttgaaaggcct
tctggcagtggaggagtctaaaaacctgaagccgagaggcagattgaatactttggctgtttccttaagtgtgcagattgtccacaaactgaaggtccgc
ttcttacaaaatgttctggcagtcagtctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaattgcagatca
aaccaagaggcaatgtctacagtggagaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaacaaaaaagcctccacttgaat
aatttagggccatgaagaaacaagctcaatgtatcatcagtcctttaggagtcagtaagatcttgcttagacctggtgttcatggatgaaaagcagcagc
caagatggaagaaccaaattgaatgttaacatttctcactgatcaagccatccttaaagaaaatcataaaaagtgttacaccatccctcaccggaaatct
agccagagccccccataccatctgggtttcattctttctccagtggaaaaaatgataattttttgaaggttcccaagtaagagttttactccaacctgaa
attcctttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: