XB-CLONE-4354473
Clone Name: IMAGE:7026705
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Gene:
mrpl24
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077060 [+]
GGCTTCCTGCACGTTGTAGTGACACCTAGGGAGCCCTTGTCTGTCTGTCCATCCCCGGGTACTGCTGACATGCGCCTGACGCTGCTTCTCGAGATGGCGG
CCAAACTCAAATTGCCCCACGATTACCGCTTTGGAATGAGCCGCCCGTAACATTGGCAGCAAAGAGGAGGAACCCCCCGGGCAAGAGAAGATCCAAAGTG
TTTGTAGAGCCCGTCAGTAAGGAGGAATGGCAGTATTTCCGTGGGGATACCGTAGAAGTTCTTCATGGGAAGGATGCTGGGAAGCAAGGAAAGGTGACAC
AAGTGGTCCGAGCTCGTAACTGGGTTGTTGTCGACGGTTTAAACACACATTACCGTTATGTTGGTCGGACAGAGGAATACAGAGGAACATATGTTGCCAG
TGAAGCCCCCCTGCTGCTCAACCAGGTGTCTCTGGTTGATCCCACAGACAGGAAGCCCACCGATATAGAATGGCGTTACACAGAGGAGGGTGATCGCGTG
AGGGTGTCTGTGAGATCTGGGCGTATCATTCCCAAGCCTGTCCTGCAGAGACGCGACGGGATCATCCCTGAGCAGTGGAAAGATGGTCCCAAGGACACCT
CTGTGGAAGATGCTCTAGAAAGGACATATGTGCCGTCCCTGAAGACATTCCAAGAAGAAATAATGGAGAAAATGGGCATCGAAGAAAACCGCCAGCAAAG
GAAATCCTACTGGTACTAACGCAAGCAGAACTGTGCGGCAGCGGCTGAGAGCGTCTCTGTCATTAATGGACCATAACAGGAATAGGAAATTGTTTGGGAA
AATTTACAGCTTCTCTGCCATTTGGAAAAACTCATGGACCTAATTCATCCTGTTTTTTTACATCATATGGGCTAGAGCTCCATATCCAGAACTAAAGGAA
ATGAGGAAATGTGCTTGCCGGTATTTATCCTGCGCAGATTGGTCACTTCCAGCCGCACCAGTACAGCCCATGTCTCCCAGTGTTGGGTGATCACAGAACG
TACGTGTTAAGGAAAGTAATAAGTACTTTTGTAATAAGAATAATCCCACCACTGAAATAAATTCAAGTATGGACAGCAAACATGTTAAAACACCAAGCTG
CAATTAAAGCTCATTTTTTTTTCCAAAAAAAAAAAAAAAA
5' EST: CF782970 [+]taattaatcccccccccccccggcttcctgcacgttgtagtgacacctagggagcccttgtctgtctgtccatccccgggtactgctgacatgcgcctga
cgctgcttctcgagatggcggccaaactcaaattgccccacgattaccgctttggaatgagccgcccgtaacattggcagcaaagaggaggaaccccccg
ggcaagagaagatccaaagtgtttgtagagcccgtcagtaaggaggaatggcagtatttccgtggggataccgtagaagttcttcatgggaaggatgctg
ggaagcaaggaaaggtgacacaagtggtccgagctcgtaactgggttgttgtcgacggtttaaacacacattaccgttatgttggtcggacagaggaata
cagaggaacatatgttgccagtgaagcccccctgctgctcaaccaggtgtctctggttgatcccacagacaggaagcccaccgatatagaatggcgttac
acagaggagggtgatcgcgtgagggtgtctgtgagatctgggcgtatcattcccaagcctgtcctgcagagacgcgacgggatcatccctgagcagtgga
aagatggtcccaaggacacctctgtggaagatgctctaaaaaggacatatgtgccgtcccctgaaaacattccaagaagaaataatggagaaaaatgggg
catcgaatgaaaaccgcccggcaaaaggaaattcctactgggtactaaaggcaaagccaaaactgttgcgggcaacccggcttgaaaaccgtcttctgtt
caattaatgggaacccttaaccaggaaaatagggaaatttgttttgtggaaaaatttttacaagcctttcctcttggccaattttgggaaaaaaccctca
ttgggaacccttaaatttct
3' EST: CF782969 [+]tttttttttttttttggaaaaaaaaatgagctttaattgcagcttggtgttttaacatgtttgctgtccatacttgaatttatttcagtggtgggattat
tcttattacaaaagtacttattactttccttaacacgtacgttctgtgatcacccaacactgggagacatgggctgtactggtgcggctggaagtgacca
atctgcgcaggataaataccggcaagcacatttcctcatttcctttagttctggatatggagctctagcccatatgatgtaaaaaaacaggatgaattag
gtccatgagtttttccaaatggcagagaagctgtaaattttcccaaacaatttcctattcctgttatggtccattaatgacagagacgctctcagccgct
gccgcacagttctgcttgcgttagtaccagtaggatttcctttgctggcggttttcttcgatgcccattttctccattatttcttcttggaatgtcttca
gggacggcacatatgtcctttctagagcatcttccacagaggtgtccttgggaccatctttccactgctcagggatgatcccgtcgcgtctctgcaggac
aggcttgggaatgatacccccagatctcacagacaccctcacgcgatcaccctcctctgtgtaacgccattctatatcggtgggcttcctgtctgtggga
tcaacccgagacaacctggttggaccaacaaggggggctttcactggcaacaataatgttccctctggaaatccctctggcccgaaaccaaccattaacc
ggtaaaatgtggtgttttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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