XB-CLONE-4354470
Clone Name: IMAGE:7026704
|
Gene:
dkc1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782972 [+]
taattaatcccccccccccccgcatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctatag
gtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagga
ggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaatg
aaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatccg
cacttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagccaatattttgagatcatcagtgaacttgaggggcaaagatcc
acgtgtgcaacttgttgtataaacgcttaccgtggacccttgcaaaattcgacaacgccaaaaactggcttggctgaacgaaatttcaaaacctaaaatt
tgtgaaggatggaacctgggcttcctttttcctcaaaaaccgtgtgaagggctggtatttttcgcctggggctctggggcggcttacgaaggtccccttc
ggagatatgtaaggactttgtaaaactaccttgggaaccacaaaaaaaaggggttttgaacccccccttatgtcgaagaaataaatgcacttattgatat
atttgcgggaagtaaattcattcttatgacaaacttcagtaattccagaagccgttgtggggggcttgtagttccctcggggatataaacaaaattcaaa
aacacccttttcctaatttgggangtggcaccaccaactgcagtgcggccctctcgggtttcattcattagtgtgctccctaataagcgtgtctcccgct
gcaaaaaaacttttgtttttatgtttttcgccgccaattggggggaaaacctcgggaatttttttttagtattcactttttgttaacttttggtatccca
ctgcttgttttggtggggggaataaactctttgntaggatgctgttgtaacttaaagtacaagaatataattgggactctctatataatttagagaaccc
gcctggggagtgtatcccccgcattttgggggggagcgcaaagc
3' EST: CF782971 [+]tttttttttttttttggtgtagaaggttctgcagaatcgctatctgtttcacttacatgcttcctctttggagcatctgctgcttgctggatttttggtg
tagcactgtaatcaacatattcctgcttccaacctgaaggtgtgctctcattaggtttgccatgcttgtctaacaagcctttctgaatcatcatcttctt
ctgactagctttaggaccaagtccccatttgcgtggataagtatccctctccataatgacacgtttaatctttgccacaactccatgatcacatgtagat
atcactgcagttgtcataagggctacggcaatacagattgcttctccttttgtagttatcaccaacaatctcttgattttagttctatcccatcctcata
tctgagcagccccggcagcatgatttttgcacccgtaacaaataccatttgactgcactaatccttcatgaaaagtctttttggtgagacacctagaaag
tttcttctagtggaaaaaatgaacctcttgcgtaaataaacttttcatcccttattgttatcaaactggcccactgcgccgtccaaaaacatctggcatt
tgtggaacagggtttatccttcttccccctaggaacacccaaaacgaaacacgaacgtaagctccctgcaattttgaacaccccaaacccccagcaatac
aaagccaaaatgtggaaccaccccaaaagtccgcggaaatatattgtacccgagccctttccaagctttaatccaaaaatataaccttagcacaaacgtt
caagggggcaaaaatccaaataagatttgccccccccgaagaattagttctttaaaaaccaaacatggctttttttaaaaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: