XB-CLONE-4354465
Clone Name: IMAGE:7026702
|
Gene:
ran
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782975
[+]
taattaatccccccccccccccccgctcgctgctttacagacgcttctggaaggctgacaaacataccatggcagcccaaggagaacctcaagtgcagtt
taagcttgttctggttggggatggtggtaccggtaaaacaacctttgtgaaacgtcacttgactggtgaatttgagaagaaatatgtagccacacttggt
gttgaagttcatcccctggttttccacactaacagagggccaatcaagtttaacgtgtgggatacagctggtcaagaaaagtttggtggtctcagagatg
gatattatatccaggcccagtgtgccattatcatgtttgatgttacatcaagagtcacatacaagaatgtaccaaactggcatagagatcttgtaagagt
gtgtgaaaacatcccaatagtcttgtgtggtaataaagtggacataaaggacagaaaagttaaggccaaatcaattgtcttccctaggaagaaaaatctt
cagtactatgatatttctgccaaaagtaactacaaattttgaaaaagccctttctttgggcttgcaaggaaaacttataggttgacccccaaactttgga
ggttttgtaagcccatggcccgaccctttggcccccaaccaaaaagggatgtaaattggaaatcccccttagatttctttagaaccttgcccgcccaacc
cgctggatgggaacttttgggcgcagttggccccaaactttttgtttgtatatagcacgcctttataanaggtggtcaaccaaaaataaaatcaatataa
gcacttatacacaaattttccaanaataaataaaggagtttttatttattgccatacagttgttaatgttgtttgggttttgtgtccaaaaatatcatat
atatgaattgttttttttcgtgaattacggatctgcgtgcaaatatctgtttcggtggcaaataccccgttgcggtgtgggaaaaaacacccctgtcaaa
ataaagaaaacatttggtgaataataacatat
3' EST: CF782974
[+]ttacaacctctggtggggcaagggctggcatggctacaaactcaagattggggtcacctataagtttccttgcaagccaaaggaagggcttctcaaaatt
gtagttacttttagcagaaatatcatagtactgaagattcttcttcctatggaagacaattgatttggctttaacttttctgtcctttatgtccacttta
ttaccacacaagactattgggatgttttcacacactcttacaagatctctatgccagtttggtacattcttgtatgtgactcttgatgtaacatcaaaca
tgataatggcacactgggcctggatataatatccatctctgagaccaccaaacttttcttgaccagctgtatcccacacgttaaacttgattggccctct
gttagtgtggaaaaccaggggatgaacttcaacaccaagtgtggctacatatttcttctcaaattcaccagtcaagtgacgtttcacaaaggttgtttta
ccggtaccaccatccccaaccagaacaagcttaaactgcacttgaggttctccttgggctgccatggtatgtttgtcagccttccagaagcgtctgtaaa
gcaaccagcggggggggggggggggggattaattaaactcgggggcaaaaagaaacaagtaaccctggtgttccgggcccggaaaaaaaccccaagtggg
ttttgggacccggggaaggggaaatccgggtggaaagaaaaggccaaaggggggaaggttattgccaattgaggcgcacccgaaagtcgttgggtgggac
tggaatacacacaggggtatgtggtcggcaatgact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: