XB-CLONE-4354460
Clone Name: IMAGE:7026700
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Gene:
ndrg1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081359 [+]
CGGGAAAAAAAAATCTGTTGAACTATCTGTAATACGGCGGCGAGAGTGCAGCATCTGGGCACCGACACATCTGGCTGGCACCCAGGGAATTCTAGCACCC
AGTGAGAAAGTGCAGCATCTGGGCACCAACGCAGACAGCGGGCATCCTATTGTACTGCTGAGAAAGAGAGCGCAGCATCAGGACACCAGGATACACTTCT
GCCTGGCACATAGAGGCTGACGGCTATGTCTGCGGAGATGACAGACCTGAATTTTGCGGAGGGGAGACCCCTGCTGGTGGATAAGGATGGCATCACGGTG
CTACTGCAGGAGTACGTCACCCAGGAACATGACATTGAGACAGCTCACGGAATTGTCCATGTCACTATGTGTGGGACGCCCAAGCTGAACCGACCTGTCA
TTTTAACCTACCATGATATCGGCTTAAACCATAAAACCTGTTTCAACTCCCTGTTTAACTTCGAGGACATGCATGAAATCACCCAACACTTCTCTGTGTG
CCACGTGGATGCGCCGGGCCAACAGGAGGGGGCAGCGTCCTTCCCAGCTGGATACATGTACCCATCAATGGACCAACTGGCCGAAATGCTCCCTGGCGTC
ATCCAGCAACTTGGACTAAAGTCCGTTCTTGGCTTGGGAATTGGGGCCGGTGCCTATATCCTTACCAGATTCGCTTTGAACCACCCCAGCATGGTCGAAG
GCCTCGTTCTGATTAATATTAACCCCTGTGCAGAAGGCTGGATGGACTGGGCAGCCACTAAGATTTCTGGATGGACCCACGCATTACCCGACATGGTCAT
TTCCCACCTCTTCTCAAAGGATGAAGTGCACAGTAACCATGAGCTGGTGGAGACCTACCGGCAGCATATAGTCCAGGACATTAACCAGAACAATCTTCAG
CTCTTCGTGAAATCATACAATAGCCGCAGAGATCTGGAGATCGAACGCCCCTTTCCAGGATCCAACACTGTTACACTAAAATGTCCTGCACTGTTGGTGG
TTGGAGACAGTTCCCCTGCTGTGGATGCAGTGGTTGACTGTAACTCAAAACTGGACCCCACTAAGACCACTCTACTAAAGATGTCAGACTGCGGGGGATT
TCCTCAGGTCGTACAGCCGGCAAAACTTGCTGAGGCTTTTAAATACTTTGTCCAGGGCATGGGATACATGCCTGCTGCTAGCATGACCAGACTGATGAGA
TCTCGCACTGGATCAGCGGCCAGCTCTTCATCCCAAGACGGCAACAGAAGTAGATCTCACACCAACGAAGGCTCAAGGAGCCGCTCCCACACCGGGGACG
GAAACAGGAGCAGAGCGCACACCGGCGACGGCAACCGCAGCCGTTCGCACACAGACAGCAACAACACCAATTCCGAGCACAACACTCCAAAATCCATGGA
AATCTCCTGCTAAGCTTGGTTCCACCTCAACAACGAAGACCTGCCTGCCAATGATAAAGATTGGAAACATCTTAACCTCCTCGCTGTCTGGGTTATTCCA
CCAGCAAACGGGGGGCTAAGGTTCGATTTAGTCTCTGTGAACTGAAACACTTGAGGACCAAAGAGTAAAAATGAAAAATAATGTATATTGTTCCAGGAAA
ATCAGACTTCAATCCATGACTAGGAGTCCAGATAAAAGTGGTATCAACTAAACCATAAATGGGTTGATTGTGCATTTAGTACCAATGGTGTAAACTCTGA
TCATTCTTTGCCTTCCCAGTCGGAAAGGCTTTACCGTGATATTTACCCATAACCACTACATTCTGAAAAAATGGCCGAACCCTTTGGGGTTTTTTTCATT
CATTCAATGTAAATATATAATGTCAGAGAGAATATTTATAAAGGCATTGAAAAAATCCTGTTTTAATAGTTGATTTACACTAGAAAAAATATTTCCTTAG
ACAAGACTCTGCCTATTTTTATGTCAAAATATATCTCAAAATTGTATTAAACGCCTTCAAAGACAAACGGATATAAAAAGTTCGATTTACAAAAAAAAAA
AAAAAA
5' EST: CF782978 [+]taattaatccccccccccccccgggaaaaaaaaatctgttgaactatctgtaatacggcggcgagagtgcagcatctgggcaccgacacatctggctggc
acccagggaattctagcacccagtgagaaagtgcagcatctgggcaccaacgcagacagcgggcatcctattgtactgctgagaaagagagcgcagcatc
aggacaccaggatacacttctgcctggcacatagaggctgacggctatgtctgcggagatgacagacctgaattttgcggaggggagacccctgctggtg
gataaggatggcatcacggtgctactgcaggagtacgtcacccaggaacatgacattgagacagctcacggaattgtccatgtcactatgtgtgggacgc
ccaagctgaaccgacctgtcattttaacctaccatgatatcggcttaaaccataaaacctgtttcaactccctgtttaacttcgaggacatgcatgaaat
cacccaacacttctctgtgtgccacgtggaagcgccgggccaacaggagggggcagcggtccttcccagctggatacatgtacccatcaatggaccaact
ggccgaaaatgctccctggcttcatccagcaaccttggacctaaattccgtttctttggctggggaattggggcccggttccctatatccttacccaaat
ttccttttgaacccccntcatcattggtcaaaggccctccctacaggaaaaaataataacctccttgttgccaaaaaagcctgggaagaggaacgaggta
aaccccccctaaagaattatcccgaggaatgggcacacccccctctttttattccacccaaaaagtgggggttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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