XB-CLONE-4354457
Clone Name: IMAGE:7026699
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Gene:
mpc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077699
[+]
CGATATCTCTGGCTGGCGTCCTCTAAAGCAGTCAGTCCCTGGTGTGTGTGACCCAGCTGGGAGTAACAGTAAAATGGCAGGCGTGCTGGCTCGAAAAGCT
GTCGACTATGTCAAGAGCAAAGACTTTCGAGACTATCTTATGAGCACGCATTTTTGGGGCCCTGTGGCCAACTGGGGACTTCCTATTGCTGCTATCAATG
ACATGAATAAATCACCAGACATCATAAGTGGACGCATGACTTTTGCCCTTACCTGTTACTCCTTGATGTTTATGAGGTTTGCATATAAGGTGCAACCCAG
AAATTGGCTACTTTTTGCTTGCCATTTTACAAATGAATGTGCTCAACTTATTCAGGGAGGACGACTGATAAAACATAAATTATCTCAAAAAGAAGCTAAA
GATAAATGAACAAAATAAGCCTTCAACCTCTGCATTTTGGAATATATGCCTCATAACACTAGGATCTTGCCAAGCAACTGTTTGCACTTTTATTTTATTA
TTTGTAGTTCAGATCAATAACCCTTCTCTTAGACATGGAAATTAATGAATTTTATTAAAAACTGTGGTTCAGGAAAAGAAGACTAGCAACATGCTGCCTG
TGAAGATGTATTCAACTAGATATATTGTGGTAGAGATAAGAGTTTGGAGTTTAATTTTGGCATCCTTGGAAATAACAGTGAAAAAGTGTTATAAATCAGG
GGCATTTATATCTGATATAGATTTTTAACCATGTAATAAAGAAAAAACATTTCTAACAAAAAAAAAAAAAAA
5' EST: CF782980
[+]taattaatccccccccccccccgatatctctggctggcgtcctctaaagcagtcagtccctggtgtgtgtgacccagctgggagtaacagtaaaatggca
ggcgtgctggctcgaaaagctgtcgactatgtcaagagcaaagactttcgagactatcttatgagcacgcatttttggggccctgtggccaactggggac
ttcctattgctgctatcaatgacatgaataaatcaccagacatcataagtggacgcatgacttttgcccttacctgttactccttgatgtttatgaggtt
tgcatataaggtgcaacccagaaattggctactttttgcttgccattttacaaatgaatgtgctcaacttattcagggaggacgactgataaaacataaa
ttatctcaaaaagaagctaaagataaatgaacaaaataagccttcaacctctgcattttggaatatatgcctcataacactaggatcttgccaagcaact
gtttgcacttttattttattatttgtagttcagatcaataacccttctcttagacatggaaattaatgaattttattaaaaactgtggttcaggaaaaga
agactagcaacatgctgcctgtgaagatgtattcaactagatatattgtggtagagataagagtttggagtttaattttggcatccttggaaataacagt
gaaaaagtgttataaatcaggggcatttatatctgatatagatttttaaccatgtaataaagaaaaaacatttctaacaaaaaaaaaaaaaaag
3' EST: CF782979
[+]ttttttttttttttgttagaaatgttttttctttattacatggttaaaaatctatatcagatataaatgcccctgatttataacactttttcactgttat
ttccaaggatgccaaaattaaactccaaactcttatctctaccacaatatatctagttgaatacatcttcacaggcagcatgttgctagtcttcttttcc
tgaaccacagtttttaataaaattcattaatttccatgtctaagagaagggttattgatctgaactacaaataataaaataaaagtgcaaacagttgctt
ggcaagatcctagtgttatgaggcatatattccaaaatgcagaggttgaaggcttattttgttcatttatctttagcttctttttgagataatttatgtt
ttatcagtcgtcctccctgaataagttgagcacattcatttgtaaaatggcaagcaaaaagtagccaatttctgggttgcaccttatatgcaaacctcat
aaacatcaaggagtaacaggtaagggcaaaagtcatgcgtccacttatgatgtctggtgatttattcatgtcattgatagcagcaataggaagtccccag
ttggccacagggccccaaaaatgcgtgctcataagatagtctcgaaagtctttgctcttgacatagtcgacagcttttcgagccagcacgcctgccattt
tactgttactcccagctgggtcacacacaccaaggactgactggctttagaggaagccagccaaagaatatcggggggggggggggggggggaaataaat
ttaaatctcgagggggcaaaaaaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: