XB-CLONE-4354452
Clone Name: IMAGE:7026694
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782983
[+]
taattaatcccccccccccccccgaaaaaacctcctctctggctctgagtagagttcttgcttcaacagtgtttgaacggaaccctctctgagtcatttt
tagaccaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcg
tcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgat
cgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaac
gtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaagac
tgtgaaccaggctttactggatctgcaccagctggcatctgacaagactgatcctcatctctgcgacttcctggaaatctgagtacttggaaggaacagg
tgaaaggccatgaaaggagctgggaagaataacattcaaaaaaccctggaaacccgcctttgggggtggcccgcaaaaaaaaggcattgtgggcaaagat
aactttggtttcgaaaaagacaaaacccctgtggggggaagaatgaaaccttaaaccgccacctctcccaggggggtggagaaaaaaaaccgaggtcaac
gttcttctgggttttcaaaaataacggggcccgcattaaaatagcggggggggggaatttaacccacataagctcccccaagggcccccggggagtgaac
cccttttttttaatcccttggggggaaaataatggggaaaaaaaacaccgggtgccctcatatacagggtgtttgggaagaacaagggacaaaaaacctg
gtacaggaggcgatgggcgaaaan
3' EST: CF782982
[+]tttttttttttttttggaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttat
tcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctg
actggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgcttgtcgaacaggtactcgcccatgccgttctgcggcaccccaaggcg
cttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttg
tcagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcac
gctctggtttcttgatatcctgaaagacggcgcggcccccacgtttgttttggtatttgaaggaacttttcggcatgctcccttttcctcatgactctgc
tccttgaaaaacttggccacatgggtggaatggccacgtcatcaccgatcgaaatagtaagacttggaacggatagacctagggaggcatacaacttcca
tgttcccccaaacggtttgaagggcggctttcccaattccctgttgaaaatttctgaacccacctggggaattgcatggcggggcgttttccgggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: