XB-CLONE-4354449
Clone Name: IMAGE:7026692
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782985
[+]
taattaatcccccccccccccgagcagtccctgtttccagaggattcatcctatctctttgctccagtttgcaaactcttacgatcagccatggcatttg
gtggactcctgagtgaggctgacatttctgctgctctacaggaatgccaagctgctgactccttcaactacaaatccttctttgccaggtctggcctgag
caaaaagtccgcagatgatgtgaagaacgtctttgccattcttgaccaggacaggagcggcttcattgaggaagatgaactgaaactgttcctccagaac
ttcagcgcaagtgccagggctttaactgatgctgaaacaaagccttcctgggtgctggtgactctgaaggtgatggcaaaaattggagttgaaaaaattc
ccggtctttggtgaaaaaataaaaaactggttgcaacttttttttttgggcaaagtttttctttcaaaacatttcaggtttccaaaaatattgggggctt
tttcccttaagtttacaaattggaaaaccctctaatggtgatgtgtgggaaaaacaacaaagaaacccccgtgtgttttaaaaaaaaattttcttcacac
ataaaacaggggggggaaanaaaaaaaagggcgcagaagaattttttcccccctattaaaagcggcgggaggagaaagagaaatattactcccagggggg
gggggttttttttttctacacacctgggacaaaaaaaaaaaaaagaaagaagggggactctccggggnatattattattttaaaaaaaaggagcgcggag
ttatattttactattgtgtggaagaaaacccccacccatttatttgttttggtggtcgccccccctattttttttctttaataaaaagaagtgtttatat
aaaaataaacacacgccccgacttttatttttatgaaaaagacacaaccatataatttttatatataagaagaagcaaaccaacccaatctagaaggcgg
ggggccttatttgtataagttaccaccacacaccaaccggagagtagtatttatcttcttgtct
3' EST: CF782984
[+]tttttttttttttttgattttaaaattctttattgatagcattgagttactttatgctttctgcctcaacctataagacatcaaaacgaaaatcaaacat
ttgtagatttacaaaagtcacagtcaatgacgtatcaatataaaaaaataatgattcataaatagacagggttaattagacaactcttagataaattgct
ggcaccaattggtggcttcagaaagtaccgactgaaaaaaacaaaaccttcctctttgcatggtaaatgccttggattgcttgcagataaggaaatcagc
atttgtccgcagtatgagaaagtctaaacaggtgtctgtgttccactacattaggtttcaatttaactaagaaaagccacaattatggaacttgatgttt
gaagaaagcttgccaaaaaaagttgcaacagttttactgtttcaccaaagactggaattcttcaactccaattttgccatcaccatcagagtcaccagca
cccaggaaggccttggtctcagcatcagttaaagccctggcacttgcgctgaagttctggaggaacagtttcagttcatcttcctcaatgaagccgctcc
tgtcctggtcaagaatggcaaagacgttcttcacatcatctgcggactttttgctcaggccagacctggcaaagaaagatttgtagttgaaggagtcagc
agcttggcattcctgtagagcagcagaaatgtcagcctcactcaggagtccacaaatgcccatggctggatcgtaagagtttgncaactggagcaaagag
ataggatgaatctctggaacagatgtn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: