XB-CLONE-4354447
Clone Name: IMAGE:7026691
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Gene:
rpl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723002
[+]
taattaatcccccccccccccgctcttcccatctccaacatggcggacaaatctgacaaagagaaccctatgcgtgagctgcggattcgtaagctctgct
tgaatatctgtgttggtgagagtggtgacagactgacccgggcagccaaggtgctggagcagctcaccgggcagactcctgtcttctctaaagcccgtta
cacagtgaggtcttttggcataagaagaaatgagaaaattgccgtacattgtacagtacgtggagccaaagcagaagagatcctggaaaagggcttgaag
gtcagggaatatgaattgagaaaaaacaacttttctgacacagggaaacttcggttttggtatccaggaacacattgacttggggattaaatatgaccca
tgcattggtatctatggattggacttctatgtggttcttggccgaccaagatttagctttgccgacaaaaaaacgcagaagaggcacatttggagcaaag
cccccgtattgtaaaagaaggaggcaatgccgctgggttccagcaaaaagttaaccgaggggcataatttcttctccttggctaagttgaaccttggttt
tacgttcttccagaaaaaaaaacatcaaattatataaatttttttccgggaataaaaacttcccattgaacgcgaaaactcattataaatggattccccc
cgtcactatttctctaaaaaggcgggctcgtgccacctggggtggttcgggagaacctttggttcccgtttggccccccccttttttgtttttttttggg
cctgtctttataagaaggggtttaacaagtataaaaaggccatttatttctattcacaaatatttttcctccaataaaaaaagcttttttatttttccaa
ctgtgcaattcattaacttgtgatgcgatttaacactataaatcttaccaccggtcgtaaccataaacccaatatcctgggatttgggggaatatatttt
ttagcttttccttcccacccattagatcccgtgaaaagtatcccttcaaggatataga
3' EST: CF782986
[+]tttttttttttttttggattttatcagaaaagttttattgatttttttctgagactgaaccagctcacttgccagggagaattatgccatcgtacttttg
ctggaaccagcgcattgcctcctcttttccaatacggtgctttgctccaattgtgcctctcttgcgttttttgtcggcaatgctaaatcctggtcggcca
agaaccacatagaagtccaatccatagataccaatgcttgggtcatatttaatccccaagtcaatgtgttcctggataccaaaaccgaagttccctgtgt
cagaaaagttgttttttctcaattcatattccctgaccttcaagcccttttccaggatctcttctgctttggctccacgtactgtacaatgtacggcaat
tttctcatttcttcttatgccaaaagacctcactgtgtaacgggctttagagaagacaggagtctgcccggtgagctgctccagcaccttggctgcccgg
gtcattctgtcacccctctcctcaacacatatattcaagcagagcttacgaatcccgcagctcacgcatagggttctcttttgtcagaattgtcccccat
gttgggacatgggacaaccgcgcgggggggggaataatttactcccttgtgcaaagaaacagttaaccctttatttccctcccggaacaaattccaattt
tcatctcgttttcggggaatgtgacctctttttcaaaaaaaaggaaaaataccgccctttaatccaccttttgtccctacataacctaaagcggttcaat
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: