XB-CLONE-4354444
Clone Name: IMAGE:7026690
|
Gene:
znf534
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782988 [+]
taattaatcccccccccccccgaatcctacaatctctagtcaagggtccccactgactgtacagctttatccaggtatccatcagaagggagaaggcaag
gtgcaggggtctaggcatgaccctacaaaccagcccaagcgagagcgctgtgtattcccagttaaaaccagtaaacatgtctgtggagggtgtggaagac
agcggtgtgtttgaggatatcaaacctgctataaggatgctggcaagtcagccggagctcagcgagcatgaaagagcagaaatggacaagtatctcctga
gccaagttcctctgcatattctgtctgacaagaagtatcggagggaaagtgcctctgttgtggatgaattctttgaagcagagaagccacaggccccgta
cagtgtaaacatcaatgttatcctgcctgatacagctcattcttcgtacaggttctctattcggccaatcctaaaaatttccactgtatctcccctcgag
gtgaaaaaataagagtcaaggccaacaaactttttgtcaaaagaccaatggcatcaagaaaaaccaatagtaaaccaagaaaatacttaccacaaaaaat
ttttaaacaaaagataggttagttttaacagttataatagtcccacctacaactattcttggattatttagctgtgtttgtattaaatataaaacgcatt
ttttgttntacccaatctaaaaacataaccaaacgtagtaaaagagagccccccctcctctttttattatctaaaagaaaaaaaataatatctccaccac
atttttctcatttttattatatatcaataggntgaagtttacatgttctttatgagaatctacccaaatattttctgatagatggaaacttcccacacga
aaccacccccctttcaagtagcaggaggttttaaacacaatataattttgccgccctaatataaataagnaagttttcccccaacagtattggggaggng
gcttctataaaatagttgtgtgtagcaaacacatcagaggaccaattgtgtgtacatagtcccttttttgtgggtacaccaccccaccaagtcatctctc
ctcataaaattattgggggataaagaaaaccacncatctcattatttttaagttgtttattgtgcgttaaaagcgaaattattgtcgcgactgttttcca
ccatttagattggaaattgaaaaaaagcacattagaatggggggaaaaaaccaaccctctccaaatgtaagttagtggggtgagtcgagagcacgctctt
tttctcctctttttttcggcccgcacgatacatactattgataagaggagaagatacctttaggaaatttaagacctcgcttctcatgctattttacatg
tttaacactctcgttggga
3' EST: CF782987 [+]tttttttttttttttctttttttatttgtatatttatttaaagtattaaaaaatgtacatatttttatttcaatctgtaaaaaaatatatatacaaacaa
aagaggttgaacgtcattgagcatcagtaaacacagatcattagtagaacaagagaatatgtagagagtaaagccaattgcgatgcagtggaacccacta
agatcagtctagtatagggaggttgaaaagaaggtcctaggtttttattttagtgcatagagatatgggcttgcgtttgaagggagtctaaaaagatgat
gcccacaagatagattttttaaataaaaaaaattacaagtatgtatcaaggaagatggcctaaatggagtgcatgttaaacatggtgtgttgtagagaat
gtatcatataaaagaaggcagatctatttggtggtccaaataaaattccacacatatatatatatatatatcgaaagggcaggatgaaagatgcaaccat
aaggagcaccaaacattttattgagtagtatcttattgtttacattaaaatgtcatttaaaaccagtgcaaatctacatagatcctttggctgctacact
gtaggggttataaaccattaaagggccacgttacccagcattggttgtagaaaataaatccatatatatatattaaagtgtggataaccgatcgcccgat
ccttgtcctcatatactcatatacagctacacagtctatatggcacaatatactgcattatgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: