XB-CLONE-4354440
Clone Name: IMAGE:7026687
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Gene:
erp44
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723004
[+]
taattaatcccccccccccccgagtaacatcaccggcatatctctatgaatatgagacgcggtatcttcgtcccttacttttgcccggcataccttctca
tgccattggtaatgtgggtttacactccagtaaaaagtgaaatacttaccctacagagtggcaatatagatgatattttaagaaatgctgatgttgcttt
agtaaatttctatgctgactggtgccgcttcagtcagatgctgcaccctatatttgaagaagcatctaatataatacaagaagaatatcctgataaaaat
aaagttgtttttgctagagtggactgtgatcaacattctgatatagcacagagatacaggatcagtaagtatcctacactaaagcttttcagaaatggaa
tgatgatgaaaagagaatacagggggcaacgctccgttactgcaattgctgactatattcgacagcaaaagagtaatcctatacgtgaaattggtgacct
ggaagaataaaaactgttgatcggagtaaaagaaatataattggcttttttgaaagcaggaaatagacaacttcagaaccttttgaaaagtagcaaatat
tttgcatgatgattgtgtcttcctcgctgcatttggtgatgtagcaaaaccagagaggtttagtggtgacaatgtaatctataaaccaattgggggagag
tgctccagacatggtttaatttgggattcattaaccaactttgacctggacattttgcttggacaccaaaataaatgtgttcccttcttgttaaaaagaa
atttcatttggaaaattggagagggaactggacgaagaaaagggccttcccgttttccaaaaacttttttccttttggaaaaatagataacgggaaagcc
ccaaaaaaac
3' EST: CF782990
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaaaaaacccc
aaattttttttggggaaaaaaaaaaaaaaaattggccccccttttttttttttgggggaaaaaaaaaaaggggggcccttttttaaaaaaaggggttttt
aaaaattaaaaatttttccagggggaaacccccaaaaaaaaaaatttttttcatttggggggggaaaaaaaaaaaaaaaaatttaccctgggggggggtt
tttcccccaaaaaaaaaaaaaaaaggaaaaaaaggggggaaattttttttccccaaaaaaaaaaggaatttttttttttttaaggggcccccggggggtt
tttttttttaaaaggtccccccccccccctttaaaaaaaggggaccctttttccctggggaccctttttggggaggggcccccggggggaaatctttttt
ttcccccggggggggggggggggggaaaacttggggggggggggccaaaaaaaaaaccccgggggggttttccaaaggggggggccaaaaaaaattggtt
aattttcccaaaaatgggaaaacctttaaggggggaaaaaaaaaccttttggcggaaaacccaggggggtataaaggggaaattccggggggttcctctt
tttgtggcaaaaaaggggggccaaaatttttcccccggcgggggggggaaaaaaaaaagggggcccctttccccccaaaaaagggggggcgacaaaccct
cttggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: