XB-CLONE-4354437
Clone Name: IMAGE:7026686
|
Gene:
rpl23a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF782992 [+]
taattaatcccccccccgccctcgaacttccttttgcccaagatggctcctaaagcgaagaaggaagctgtccctcctaagattgaagcaaagtctaagg
ctctcaaggccaaaaaggctgtgttgaaaggagtccatagtcacaagaaaaagaaaataaggacctcaccaaccttcaggagaccaaaaacactcagact
gagggaggcaacccaaatatcctagaaagagtgcccccacaagaacaagcttgatcattatgctattatcaagtttcccactgaacaactgaattccgcc
cttgaagaaagaatcgaagaacaaacaataactctgggtttttcatttgttggaagggttaaagccaaaaccaagcccccccagatctaaaaaaagggcg
gttgggaaagaaaaacccctatatgaaaatttgtggggtttaccaaaaaagggggaaaacacccctttttattcaaaggccccctcaaatggggttgaaa
aaaagaaaaaaggcgcttaatttgttcccccccctttatgccttccccaaaaaatattttaaaaagagcgccttttgtgggagaaagagttgtgggacca
aaaaacaaacaaataaatattgtgggggcctactccttactctttaaaagaacaaacaccgggcgagaattttttttttaaaaaaaaaaaaaaaactttg
ttttctcacaaaaaaaaaaaaaaaaaaaaggagggggggtgtgttttctcttcttccgcggccccccaaaaaantggnngnatacataatataanaanat
ggggggggggtgcgtctcccccctctctgatttgttctctcttaaaaaagagggggcggggcgcgccccccactccacacacnggtgcggtggggagaat
tattattggttggggctggggtggccccgcccacaaattttttttgtttttttttttgccgcgcccctcctataaataaggggggggcgagggcaccaaa
caaaaagaggggcggtctctttataactccttccacacaacaattta
3' EST: CF782991 [+]tttttttttttttttaggcagaaaaacacatttatttctgtacagctttttgaagatgcaggtttagatgatgccaatcttgtttgcaacatccaaagca
tcataatctggagctaggcggacgtatgctttcttttcaccatcaggcctgatcaaggtgttcactttttgtacatcaatgtcatagagtttcttcactg
cctgtttgatctggtgcttgtttgctttaacatcaacaatgaaaaccagagtattgttgtcctcgatcttcttcatggctgactcagtggtcagtgggaa
cttgataatagcataatgatcaagcttgttccttgtgggggcactctttctaggatatttgggttgcctcctcagtctgagtgtttttggtctcctgaag
gttggtgaggtccttattttctttttcttgtgactatggactcctttcaacacagcctttttggccttgagagccttagactttgcttcaatcttaggag
ggacagcttccttcttcgctttaggagccatcttgggcaaaaggaagttcgagggcgggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: