XB-CLONE-4354435
Clone Name: IMAGE:7026685
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Gene:
gsto2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077010
[+]
GAGCTTTGTTTCCCTTTCCCTGCAGGGTGGTTGTACCTTCTGTGTATCTGCTCGTACTGTCAGGCTGCTGAAATGACCGGATCGGAGAAGAGTTTGGCCA
AAGGCAGCCCAGCTCCGGGCCCAGTCTCTGAAGAAACAATTCGTGTGTACAGCATGAGATTCTGCCCATATGCTCAGAGGGCAAGGCTTGTTTTGGCTGC
CAAAGGAATCAAGCATGAAGTAATAAACATCAATTTGAAGAACAAACCAGACTGGTTCATTGAGAAGAGCCCATTTGGCCTTGTTCCATCTTTGGAGACT
AGCAGTGGTCAGGTGATCTATGAATCTCCAATCGTTTGTGATTATCTGGATGAGGTTTACCCTGGGAAGAAGTTGACCCCTGTAGACCCCTTCCAGAAAG
CCCAACAAAAGATGATAGTGGAACATTTTTCCAAGATCTCAACATTGTTCTATAAAATATTGTTAGCCAAGAAAAATAATGAAGATGTATCGGGTGTGAA
AGCAGAGGTCCAGGAGAAGTTGGTTAAACTTGATGAGATCCTGGCTAAACAAAACGGCCTTTTCTTTGGTGGAAGTGATGTTTCTATGGTTGACTACATG
ATCTGGCCATGGTTTGAACGGCTCATCATTTTCGATTCAAAGGATTGTTTGAATAAAACCCCACACATTGATAAGTGGTATCAACAGATGCTGCAGGATC
CAGCAGTCAAGGCCACATATATTGAACCAGATCTCCTGTTAGGCTTTTTCAAGCTATACAGCCAGAATGATGTGGAAGCCTGTGATTATGGTCTCTAACA
CTTTCTTGTAATATACAGTACCTTGTTGCTGACTGGGAAATTCTTTTGCACTTTGCTGTAACTATTTTTTTAAAACCCAATAAAGGTGCCTTTCAGCCTG
CTAAAAAAAAAAAAAAAA
5' EST: CF723005
[+]taattaatcccccccccccccgagctttgtttccctttccctgcagggtggttgtaccttctgtgtatctgctcgtactgtcaggctgctgaaatgaccg
gatcggagaagagtttggccaaaggcagcccagctccgggcccagtctctgaagaaacaattcgtgtgtacagcatgagattctgcccatatgctcagag
ggcaaggcttgttttggctgccaaaggaatcaagcatgaagtaataaacatcaatttgaagaacaaaccagactggttcattgagaagagcccatttggc
cttgttccatctttggagactagcagtggtcaggtgatctatgaatctccaatcgtttgtgattatctggatgaggtttaccctgggaagaagttgaccc
ctgtagaccccttccagaaagcccaacaaaagatgatagtggaacatttttccaagatctcaacattgttctataaaatattgttagccaagaaaaataa
tgaagatgtatcgggtgtgaaagcagaggtccaggagaagttggttaaacttgatgagatcctggcttaacaaaacggccttttctttggtggaaatgat
gtttctatgggttgactaacatgatctggccatgggttttgaacggcttcatcatttttcgaattcaaaggaattgttttgaaataaaaaccccaccaca
ttgataaaattggttatcaacaagatgcgctgcgagaatccaccccgtccaaaggccaaattattttttggaaccaagattcccccctggttaagggctt
tttttccagcgcatattctagccccgaaattgaatggggggaaaaccccggttggtattttggggtcctcctaaaacaacttttttttgttttaatttaa
ccaagtaaccctctggtttatgttaccaactgggataaataatattttttgccc
3' EST: CF782993
[+]tttttttttttttttagcaggctgaaaggcacctttattgggttttaaaaaaatagttacagcaaagtgcaaaagaatttcccagtcagcaacaaggtac
tgtatattacaagaaagtgttagagaccataatcacaggcttccacatcattctggctgtatagcttgaaaaagcctaacaggagatctggttcaatata
tgtggccttgactgctggatcctgcagcatctgttgataccacttatcaatgtgtggggttttattcaaacaatcctttgaatcgaaaatgatgagccgt
tcaaaccatggccagatcatgtagtcaaccatagaaacatcacttccaccaaagaaaaggccgttttgtttagccaggatctcatcaagtttaaccaact
tctcctggacctctgctttcacacccgatacatcttcattatttttcttggctaacaatattttatagaacaatgttgagatcttggaaaaatgttccac
tatcatcttttgttgggctttctggaaggggtctacaggggtcaacttcttcccagggtaaacctcatccagataatcacaaacgattggagattcatag
atcacctgaccactgctagtctccaaagatggaacaaggccaaatgggctcttctcaatgaaccagtctggtttgttcttcaaattgatgtttattactt
catgcttgattcctttggcagccaaaacaagccttgccctctgagcatatggggcagaatctcatgctgtacacacgaattgtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: