XB-CLONE-4354432
Clone Name: IMAGE:7026684
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Gene:
itln2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077059 [+]
GGACATCCCCAGAAAAAGGGCAGCCTCTGAAATGGGCAAGATGTTGGTATATAGCCTGTTGGTTTTTGCCCTTGCATTTCCTGCAGGGCACGCTGGTTCA
TGTGAACAAGCTTCAATTTCTGAAAAGAAGGAAAACATTTTGAACCTTCTGGCATGCTGGACAGAGGATAAGACAGACAACAGCAGGTCCCGTTTCAGTG
GCTCTCCAACTGGTGATATGAGCTATGGATACAGGAGCTGCAATGAAATCAAGAGCTCAGACCCATCAGCTCCTGATGGCATATACACACTGGCCACTGA
GGATGGTGAATCCTATCAAACCTTCTGTGATATGACAACTAATGGGGGTGGTTGGACCTTGGTGGCCAGTGTTCATGAGAATAACCTGTACGGGAAGTGC
ACTGTGGGGGATCGCTGGTCCACACAGCAGGGAAACAATATACAAAATCCAGAAGGTGATGGAAACTGGGCAAACTATGCCACCTTTGGGCTACCTGAAG
GAGCTACAAGTGATGACTACAAGAATCCTGGCTATTATGACATCCAGGCTAAAAATCTGGCTTTGTGGCATGTGCCCAACAACACTCCAATGGTTAACTG
GAGGAATTCATCAATTCTGAGATATCGCACACAGAATGGATTTTTTACTGATGAGGGAGGAAATTTGTTTCAACTCTACAAGAAATATCCAGTAAAGTAT
GATATTGGTAAATGTCTAGCAGACAATGGACCCGCTGTGCCTGTAGTCTATGACCTTGGCAGTGCAGAGAAGACTGCATCTCTCTATTCACCAAAAAAAA
AAAAAAAA
5' EST: CF782995 [+]taattaatcccccccccccccggacatccccagaaaaagggcagcctctgaaatgggcaagatgttggtatatagcctgttggtttttgcccttgcattt
cctgcagggcacgctggttcatgtgaacaagcttcaatttctgaaaagaaggaaaacattttgaaccttctggcatgctggacagaggataagacagaca
acagcaggtcccgtttcagtggctctccaactggtgatatgagctatggatacaggagctgcaatgaaatcaagagctcagacccatcagctcctgatgg
catatacacactggccactgaggatggtgaatcctatcaaaccttctgtgatatgacaactaatgngggtggttggaccttggtggccagtgttcatgag
aataacctgtacgggaagtgcactgtgggggatcgctggtccacacagcagggaaacaatatacaaaatccagaaggtgatgganactgnngcaactatg
ccacctttgggctacctgaaggagctacaagtgatgactacaagaatcctggctattatgacatcccagctaaaaatctggcttttgtggcatgtgccca
naacacactcccaatggttaaactgggaggaattcatcaatttctgagatatcgcacacaggaatggattttttactgatgaggggaggaaatttgtttt
caactctacaagaaatatccaagtaaagtatgatatttggtaaatgtcctagcagacaatggaccccgctgtgccctgtaatctatgaaccttggcagtg
cagagaaaacttgcatctctctatttaccaaaaaaaaaaaaaaaaggaatcccctagtttctagaaccggccgccaacccccggtggaaacttgggccct
tggccccaaccttggtttatttgt
3' EST: CF782994 [+]tttttttttttttttggtgaatagagagatgcagtcttctctgcactgccaaggtcatagactacaggcacagcgggtccattgtctgctagacatttac
caatatcatactttactggatatttcttgtagagttgaaacaaatttcctccctcatcagtaaaaaatccattctgtgtgcgatatctcagaattgatga
attcctccagttaaccattggagtgttgttgggcacatgccacaaagccagatttttagcctggatgtcataatagccaggattcttgtagtcatcactt
gtagctccttcaggtagcccaaaggtggcatagtttgcccagtttccatcaccttctggattttgtatattgtttccctgctgtgtggaccagcgatccc
ccacagtgcacttcccgtacaggttattctcatgaacactggccaccaaggtccaaccacccccattagttgtcatatcacagaaggtttgataggattc
accatcctcagtggccagtgtgtatatgccatcaggagctgatgggtctgagctcttgatttcattgcagctcctgtatccatagctcatatcaccagtt
ggagagccactgaaacgggacctgctgttgtctgtcttatcctctgtccagcatgccagaaggttcaaaatgttttccttcttttcagaaattgaagctt
gttcacatgaaccagcgtgccctgcangaaatgcaagggcaaaaaccaacaggctatataccaacatcttgcccatttcagagctgcctttttctggag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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