XB-CLONE-4354427
Clone Name: IMAGE:7026681
|
Gene:
foxp4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080172 [+]
TTGTATTTATTTTGGTATGGATACTTTTCTGTACAGTTACTTTAGCCCTCTGAACCCAGGGTGTGAAGAAGCATGAGGGTGATCGCTGAACCCCCTTATG
GAAGTCACCTAGGAAGCCAATGCTGATCGTCACCTAGGAGCCCAATAGTCCGGACTCATAACTCATACCGTCGCTATCTGACAATCAAGAGAAGATCCGC
CCCACTCCAGAGACTTTCTGCCTAATAGCCCTACTCCCAGTCCAGGGTCACAAAATCAAGTCATGATGGTGGAATCTGCATCTGAGTCCATACGGTCCAC
CCCAACCAGCCAAAATGGAGTTGGAAGCCTCCCTAACCAATCAGAGAGTTGCGTGGGAAGAGAAGGAGCAGGCAGTGGTGACACTAATGGAGAGTTAAGC
CCAGCAGAACTTCTGCACTTTCAGCAACAGCAGGCTCTGCAGATGGCAAGGCAACTTCTTCTTCAGCAAGCAACAGGTTTGAGCTCCCCCAGTAGCACAG
ATAACAAGCAGCCCTCAGTACAGGTCCCAGTCTCAGTTGCTATGATGTCCCCTGGAATGATCACCCCTCAGCAAATGCAGCAGATTCTGTCCCCTACACA
ACTCCAGGCTGTCTTACAGCAGCAACAGGCACTCATGCTACAGCAGTTGCAGGAGTATTACAAGAAACAGCAGGAACAGCTGCACCTTCAGCTTCTGTCT
CAACAGCAGGCTGGGAAGCAACAACCAAAAGAGTCATCTTTGGGAAACAAGCAGTTGGCTTTCCAGCAGCAACTATTACAAATGCAACAGTTGCAGCAAC
AGCACTTACTAAATCTCCAGAGGCAGAACCTGGTGGGGCTTCAATCTGGGCAAGGGCCATTACCAATACAGAGTCTTCCACAAGCTGTATCTCCGTCAGA
CCTGCAGCAGCTCCTAAAGGAGATGAGCAGCAATCAGGAAGAATCTAGTAAACAGGACACTGTTGACCTGACCACCTCAATCACTACCTCATTCCCAAAT
TCCAAGGTTTCACTTCCTACAATACACCCCTCCTTACCTAATGGGCAGAACACACGCAGAGATAGCATGTCACATTATGAAAGTTCTCACCCACTCTATG
GTCATGGTGAATGCAGATGGCCAGGCTGCGAAGCTCTCTGTGAAGACATGGGACAATTTATAAAGCATTTAAACACAGAGCATGCTCTTGATGATCGCAG
CACTGCACAGTGTCGAGTTCAGATGCAGGTGGTGCAACAGCTTGAGATACAGTTGGCAAAGGAGAGTGAGCGATTGCAGGCAATGATGACGCATCTTCAC
ATGAGACCATCAGAACCAAAGCCCTTTAGTCAACCACTCAACTTAGTATCCAGTGCTTCCCACAACAAAATGTCCCCTGACACATTCCCAGATGGCTTAC
CTCAACCCCCTACATCTGCTACAGCCCCTATCACTCCACTGAGACAGGGCACCTCTGTGATTAGCTCCTCAAGTCTGCCTTCTGTCGGTCCTGTGCGTCG
AAGGATTGTAGACAAGTTTAGCACCCCAATATCATCAGAACTTGCTCAAAATCATGAGTTTTATAAGAATGCTGAAGTACGTCCTCCATTCACATATGCC
TCTTTAATCCGACAGGCTATATTAGATACACCAGACAGACAGCTGACATTAAATGAGATTTACAACTGGTTCACGCGCATGTTTGCGTACTTTCGGAGGA
ACACTGCTACATGGAAGAATGCAGTTCGTCACAACCTGAGTTTGCACAAATGTTTTGTGCGTGTGGAGAATGTGAAGGGAGCAGTGTGGACAGTAGATGA
GTTGGAATACCAAAAGAGAAGGCCGCCAAAGATGACTGGTAGCCCGACCCTTGTTAAAAACATGATCTCTGGGCTGGGATACAGTGCCCTAAATGCAAGT
TACCAGGCTGCCCTGGCAGAAAGTAGCTTTCCATTGCTCAACAGTCCACCACTACACAACAGTTCAGGCAGTGTCCTCCATGGTGGGCATGACGATGTTA
CCAGCACCGGGGAACCTGGAAACAGCAATGGAAGCAGCCCTCGTCTGTCTCCTCAGTATAGTCAGTCCATCCATGTCAAGGAGGAGCCTGCAGAAGATGA
TGTCAGACCTGCCTCTCTTTCTGCACCAACTAATCAAACTACAGTCCTGCCAGAAGATCGTGACATAGAGCCAGAGACACCCATGGAAGACTTGCCTTAG
TGGCACCAAATTTCATAATTTTCATTGTCCCAAAGTCCAGCACTTCCACCAGAGACCAACCTGTCCCAAAGCCTCATCTACTCTCAAAGAGACTTGAACT
TGATTCTTATACAAACTTTGGGGCCAACCATAATCTGGCAGCACTTCCATGGTTTATGGACTGGAGGGAAACTCCCAGAATCATTTGGCTCATGGACAAA
GATGGGATGGGTATTGATTCTTCTAGTGACCTCTGACATTGTCAGGAATAAAGTTCCAATCCTGTGTTCTCGCCCCCACCACTGCATAATATGGAAATAT
GGACATATTTTGCCATTGCAAACTCCATGAATATTCCCTTACAATGCATGCAGGATTGGGGGTCCCTTATGCTAGCTCTTCTGCTGGTCAATTTTGAAGG
TACCAAAAATAACCAAACTTTGTTATTTAAAAAAAAAAAAAAAA
5' EST: CF723007 [+]taattaatcccccccccccgccttgtatttattttggtatggatacttttctgtacagttactttagccctctgaacccagggtgtgaagaagcatgagg
gtgatcgctgaacccccttatggaagtcacctaggaagccaatgctgatcgtcacctaggagcccaatagtccggactcataactcataccgtcgctatc
tgacaatcaagagaagatccgccccactccagagactttctgcctaatagccctactcccagtccagggtcacaaaatcaagtcatgatggtggaatctg
catctgagtccatacggtccaccccaaccagccaaaatggagttggaagcctccctaaccaatcagagagttgcgtgggaagagaaggagcaggcagtgg
tgacactaatggagagttaagcccagcagaacttctgcactttcagcaacagcaggctctgcagatggcaaggcaacttcttcttcagcaagcaacaggt
ttgagctcccccagtagcacagataacaagcagccctcagtacaggtcccagtctcagttgctatgatgtcccctggaatgatcacccctcagcaaatgc
agcagattctgtcccctacacaactccaggctgtcttacagcagcaacaggcactcatgctacagcagttgcaggagtattacaagaaacagcaggaaca
gctgcaccttcagcttctgtctcaacagcaggctggggaagcaaccacccaaaagagtcattctttgggaaacaagccagttgggcttttcaggcaccaa
ctatttacaaatggcaacaggttggcggccaaccggccctttactaaaatctccccggagggcgaa
3' EST: CF782998 [+]tttttttttttttttaaataacaaagtttggttatttttggtaccttcaaaattgaccagcagaagagctagcataagggacccccaatcctgcatgcat
tgtaagggaatattcatggagtttgcaatggcaaaatatgtccatatttccatattatgcagtggtgggggcgagaacacaggattggaactttattcct
gacaatgtcagaggtcactagaagaatcaatacccatcccatctttgtccatgagccaaatgattctgggagtttccctccactccttaaaccatggaag
tgctgccagattatggttggccccaaattttgtataacaatcaagttcaagtctctttgagagtagatgaggctttgggacaggttggtctccggaggaa
gtgctggactttgggacaatgaaaattatgaaatttggtgccactatcgcaagtcttccatgggtgtctctggctctatgtctcgatcttttggctggac
tgtagtctaattagttggggcagaaaaaaaggcaggtctgacatcatcttctgcgaggtcctccttggcatggattggactgacctatactgaggagacc
acaggagggccgccccccttgcctgtatccaggttccccgggtgcgggaaaaattgctccggactcctgtgggaggaagactcccttaacctgctgtgta
agtggatagaccttttcacaaaatggcaaaaactacttttcgggccggaggtaatcctgcttaatcttgcacccgatgggaaacgttgattcccatgcaa
aataattcacggtttttttaaaaatggtcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: