XB-CLONE-4354420
Clone Name: IMAGE:7026678
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Gene:
ckb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783002 [+]
taattaatcccccccccccccgacacatcccttcaggtctggggcaagtgttggtcctgaggagaggttagggtctccagtttgcggttttcaattaagc
aagactaagcaatatcagtatggcgcaaccaattattaagaggttgtcccttgaggaagaataccctgatctgagcaaacacaacaaccacatggctaag
gtgcttactgtggatatgtacaagaaactgaggagcaggatgacttccagtggattcaccctggatgatgtcattcagactggggttgacaatcctggcc
atccctttattatgacagtgggatgtgtcgctggggatgaagaatcttatgaagtctttaaggagctctttgaccctatcattgaggacagacatggtgg
ctacaagccaacagatcagcacaagactgacataaattcagaaaacctaaagggaggtgatgatctggacccaactatgtactcagctctcgggtcaaaa
ctggaaggagtattcgtggattcagcctcccacctcactgcagccgtggaaaaaagcgtgcaattgaaaagatgtcaattgaagcactttgccagcttgg
agggggacctaaaaggaaaatactacgcttcttaaaccatgaccggatcaggaaccaacaccagcttattcaatgaccactttccctgtttgataacccc
ggtttcccccccctcctccttggccctctggaattgggcccgtggaatgggccctggatgcccagaaggaattttggggccacaatcggacaaacaaaga
acctttcctttgtggtttgggattacaagttgagggaaaaaaccagtctcttcgcggggggggattcccccaaagggccaaaaaaagggggtgggtgcta
accaattgtatagggg
3' EST: CF783001 [+]tttttttttttttttgcctttgacaggtcaacgcaagaccattgtttattttctatctttagtaacattgcagccatcaaaagaaagataattctaagaa
acagatggaagacatttctacagtgttactaagcaggttgggtccccagggtcttggagtgtagctatttcatccaataaattagaaagcatggccaata
aagtgcagttatttctgtgctggaatgaggtcatcgatggactctcctttttccaggcgtttttccatttcaacaaggagttttacaccatcaaccacca
tttggaccagttcaacctcagagaaacccagacgatcagcattggagatatcaaacaccccaccaacggctgcagtgtctacaccacctgttcctctctt
ctgtagtctaagtctcttcaggatttcaccaaacttctcattcttgcttaagtttggcaacttaatgtgaacaccagccctcagaccagtaccgaggttg
gatgggcaggtaagaacataaccaagatgctggttccacatgaatgcagatcctttgttcttgaagatagcttcaatctttgtaaggccagtgcaaaacc
tctggaacacgtccttcatgttgccacccttttgcattgagatcacacgaagatggtcttcctcattgatccagacaaggaaggtcttgttgtcgttgtg
ccagattcctctggcatcaggccagtcacgggccattccagaagcccaagaagagtggaagaaactggcttattcaacaggaaagtggtcatcn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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