XB-CLONE-4354415
Clone Name: IMAGE:7026673
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Gene:
rpl17
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783005 [+]
taattaatcccccccccccccgctcttcctgcgcggcctgagattctctcggaacaatggtgcgctattctctcgacccggagaaccccacaaaatcatg
caaggcacggggctcaaaccttcgagtgcactttaagaacactcgtgaaactgctcaagctatcaaaggcatgcacatccgtaaggccaccaaatacctg
aaaagacgtcaccctgaagaagcagtgcgttcctttccgccgctacaacgggggtgttggaaggtgtgcccaggctaagcagtgggactggacactggga
cgttggccaaaaaagagcgcaaacttcctcctgcacatactgaaaaatgcttaaagcaatgcaaaattcaagggtttggatgttgattcattggtcattg
aacacatccaggtgaataaggcccccaagatgcgcaggggtacactaccgtgttcatggtcgtaaccatccctatattgacctcctcatggccaaatggg
aataggattccttaaatgaaaaaagtatttatatcggtacccacacacccttgaaaggaggaaaaaaccagctcaaaaaaaaaaaaaaggatcctctccc
aaaaaaaaaagcgtggaagaaatctccgaaaaacacctccttgtgagatacgggccgataataacagtgacctatcccccttagtcccgaggttagaaac
attattacgccgctcgcgcttgaaaatatatacggtgggtgtgtacatcgacgatgaataaattcaaagaaaatatgccatggagagaaataaagatggt
tggggggggtgcttttgccgggctcctctaaaagagaaatcctccgaacaaaaaaaaagaagagcgatngaaaagctactgacacctttttacctaaccc
tctactcaaccttatagggagtaaaagaatgtctgggtgggggccgccgccctgcgttttggatgtacacacacnaagaagttctgagaaagttttactc
agattaaataggtgaaatagatcccttatttataaatataatanggagattntataatgcgaaacaactaaatataatatgtctattaattttggagcct
ttttttttaacactgcaataatttttttttttatcggaagaacaaatcgtagaaaagatgaagagtagtgtatatgttttaatggcgcccggggtcttgt
attctacatatgatagaangttttgtagttcctcctgtcaaccactctatagattgttactacctagggggaggtggaggtgtccttaatattcttttcg
tgatttagtgggggatagaaggaggaaatttaaatttttttgactatctcttatagacacacgagtgtggcccttgtgacatancttttatctttttgga
aaaaaaaaagatncacatgcagtagatatatag
3' EST: CF783004 [+]tttttttttttttttctttttttttcagtttttttgggactttattntctgtacaggttgtatttattcccgagccatgagtttctgctttttcagcttc
ttctgagagatctttttcttttgagcgacttcctcctcaggttttggtacgatctgttccttctcagtaaggatcaattcgatgtggcatggggagctca
tatagggattgatacgaccatgagcacggtaggtacgcctgcgcatcttgggggccttattcacctggatgtgctcaatgaccaaagaatcacatcccaa
ccccttgacttctgcattgcttttcagcatttttcagtatgtgcaagaggaaagtttgcgcccttttttgggcaaccgtccccagggcccagtcccactg
gcttaacccggggcaaacctttcaaaacccccccctttgaaccggcggaaaaggaacccccctggttttctttaggggggaaacctccttttccggggat
ttttggggggcccctttaccggaagggggcccgggcctttttaaaaaaccttggggccaattttttccccgagggtgtttctttataaaggggccctccc
caaaagggtttggaagacccccccgggcccctttgaagaaaaattttttggggggggtctttcccggggggtccagaaaaaaaaaaaccccccccccttt
tttttttccaaaaaaaaaaatttctcggggccccccccacaaaaaaaaacagggggggggggggggggggggaaatataaataaacacccccgggggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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