XB-CLONE-4354407
Clone Name: IMAGE:7026667
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Gene:
eif4g2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783010
[+]
aaaactgcgagtacctggggaaaaccccagggcctggcgttcaaagatgggttccttcacgaagcactaaacgagatgtcaactccacaaacaactccgc
aaacactgcgatcagctcaagtgagaaagaacgacacgatgcgatctttaggaaagtgagaggcatcttaaacaagcttgctcctgagaagtttgacaag
ctatgccttgagctcctcaatgtgggtgtagataccaaactcatccttaaaggagtcatactgcttattgtagacaaagcccttgaagagcccaagtaca
gctcactctatgctcagctatgtctgcgattagcagaagaggcaccaaactttgacggtccgtcaactgacggtcaaccagggcagaagcaaagcacaac
gtttagacgcctcctaatatcaaagcttcaagatgaatttgagaacagatccagaaatgttgaattatatgacaagcgggacagcccactccttcctgag
gaagaggagcagagagccatcgctaagattaagatgctgggaaacatcaaatttattggagagcttggcaagcttgacctcatacatgaatctatccttc
ataggtgcatcanagcacttnttggaaaagaagaagagagtacaacttaaggatatgggggaaagatttggagtgtctctgtcagataatgaggactgtg
ggtccaagattagaccatgaaaaagcaagtccttgatggatcagtactttgcccgtatgtgcgctttgaaggcaaataagaggtgcaagcaaggattcgt
tttctgctgcaaaatactatgggagttgcgaagaaaacacttggggtttcctcgcaaagcttttcattgacaatggaaccaaaggacaatcacttcaaaa
tccgccgaggatggcaggtaaaagattctagggagtccttt
3' EST: CF783009
[+]tttttttttttttttaagtatcacaatgtttattgatagatacaagtatataaaaccagggcatgaacatgacttgataaattaagcagacttcattcca
atactataataaggaccaatttccatttctcaccatttttgttgaacgtttcacacccacaataccacgcgtttcagggcatcgattttttcgttttcga
tttttatttttgtgcaagtaaaccatgttttttttttgttttttttgtttccgaaagacttgtgcactcgcccaggatcggagatattaaaacctagcac
agaagcccatgactagaggtagaaatacaggcaaatatactattacggcaacgaccaatcacttacagttataggagtctgtaaccaaaatggataatat
tgcgacgactcaagtgtgacccccgagcaaagtgcgtctctacagtattcagtgttgctagggcgaagggaaagcggcggcgacggccgagtcagtcctt
gcaattgacccacttcgattggaggacgtgtgtgatgtcactattgcatgctgctaataggctaaacgtgaaaaaaaaacgctaaaaaatatcaaagatg
gccgactatgattaagacatgacactaaaaccctctggcggaagggaaatcctactgaacgcgcccttcaacatcgggacgtttgccgaaaaatggttac
cggcgggaaagggatttttttccccagtggggtttaaaagggtccaaaaaaaaaaagggaaaaaaaaaaaatggaaaaaaaaggtgggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: